工业酵母菌株转化体系的建立  

Development of transformation systems for the industrial yeast strains

作　　者：王颖[1] 鲍晓明[1] 杨国梁[1] 史文龙[1] 白凤武[2] 曲音波[1] 

机构地区：[1]山东大学微生物技术国家重点实验室,济南250100 [2]大连理工大学生化工程研究所,大连116012

出　　处：《工业微生物》2004年第1期6-11,共6页Industrial Microbiology

基　　金：国家自然科学基金委员会;中国节能投资公司联合研究基金资助项目 (No .5 0 2 73 0 19)

摘　　要：本文通过敏感度实验测定了工业酵母菌株SK 1、SPSC 1、NAN 2 7在不同的培养基上对G4 18及Cu2 + 的敏感浓度 ,确定了 3株工业菌株转化子的筛选方法。针对它们各自的生理生化、遗传特性 ,用改进的醋酸锂完整细胞转化和原生质体转化两种方法 ,分别建立了 3株菌的转化系统 ,初步解决了工业酵母菌株转化的问题 。The sensitivity of the three industrial yeast strains,SK-1、SPSC-1 and NAN-27, to G418 and Cu 2+ on different media was determined by the sensitivity test.The result enabled selection of the transformants.According to the different growth characteristics,physiological characters and genetic background of the three industrial yeast strains,LiAc transformation method and spheroplast transformation method were modified,and the transformation systems for the three industrial yeast strains were established.The development of the transformation systems for the three industrial yeast strains is essential for improving the yeast strains by metabolic engineering.

关 键 词：工业酵母菌株 显性选择标记 转化体系 营养缺陷型 

分 类 号：TS261[轻工技术与工程—发酵工程]