下颌下腺脱细胞载体的制备及超微结构观察  

The development and ultrastructure study of acellular submandibular gland scaffold

作　　者：邓春富[1] 刘月[2] 谭学新[1] 周青[1] 黄绍辉[1] 王玉新[1] 许涛[3] 

机构地区：[1]中国医科大学第一临床学院口腔颌面外科,辽宁沈阳110001 [2]中国医科大学口腔医学院,辽宁沈阳110002 [3]鞍山市中心医院口腔科,辽宁鞍山114000

出　　处：《上海口腔医学》2004年第1期48-50,共3页Shanghai Journal of Stomatology

摘　　要：目的:制备天然的下颌下腺脱细胞载体材料,观察脱细胞后的超微结构变化。方法:采用化学除垢剂法,对SD大鼠的下颌下腺进行脱细胞处理,通过透射电镜及扫描电镜对细胞载体进行观察。结果:透射电镜下见,下颌下腺细胞溶解破坏,只残留一空虚的陷窝。扫描电镜显示,正常的下颌下腺细胞呈球状,表面略凸凹不平。而脱细胞处理后,细胞形态消失,胶原纤维交叉排列,呈网状结构。结论:TritonX-100脱细胞处理的下颌下腺细胞成分完全消失,而胶原纤维为主的基质成分排列正常。PURPOSE:To develope nature acellular submandibular gland scaffold materials and observe its ultrastructures. METHODS:The fresh submandibular glands of SD rats were cell-extracted by chemical detergent and observed with transmission and scanning electron microscope. RESULTS:Submandibular gland cells were dissolved and destroyed under transmission electron microscope .Normal submandibular gland cells were ball, but the shape of cells disappeared after cell-extraction under scanning electron microscope. Collagen fibers were arranged as net structure .CONCLUSIONS:Submandibular gland cells disappeared and the arrangement of collagen fibres were normal by Triton X-100 cell-extraction.

关 键 词：下颌下腺 超微结构 组织工程学 脱细胞载体材料 制备方法 化学除垢剂法 

分 类 号：R78[医药卫生—口腔医学]