表皮生长因子对下颌下腺导管细胞内钙和环磷酸腺苷浓度的影响  

Effects of epidermal growth factor on calciumion and cAMP in the ductal cells of submandibular glands

作　　者：黄绍辉[1] 寇育荣[2] 谭学新[1] 周青[1] 邓春富[1] 王玉新[1] 

机构地区：[1]中国医科大学第一临床学院口腔颌面外科,辽宁沈阳110002 [2]中国医科大学第一临床学院口腔内科,辽宁沈阳110002

出　　处：《上海口腔医学》2004年第1期56-58,共3页Shanghai Journal of Stomatology

基　　金：辽宁省教育厅科技攻关计划(202013148)

摘　　要：目的:研究表皮生长因子(EGF)对体外培养的下颌下腺(SMG)导管细胞内钙(Ca2+)、环磷酸腺苷(cAMP)浓度的影响。方法:在SMG导管细胞培养液中加入EGF后,以Ca2+结合荧光染料(Fluo-3)及125I-cAMP放射免疫试剂盒,利用荧光显微镜及放射免疫方法检测EGF对细胞内Ca2+、cAMP浓度的动态变化。利用SPSS8.0软件,对所获得的数据进行统计学处理。结果:加入EGF后20s内,细胞内Ca2+浓度可迅速达到峰值,然后缓慢下降,维持在较高水平,作用时间持续5min,与静息状态下相比,仍有显著性差异(P<0.01);给药后3min时,可引起细胞内cAMP值快速降低;而作用90min后,cAMP浓度可恢复至基础水平。结论:EGF可能是通过调节细胞内Ca2+和cAMP的浓度发挥促增殖作用。PURPOSE:To study the effects of EGF on Ca2+ and cAMP in the ductal cells of rat SMG. METHODS:Fluorometric technique and radioimmunological technique were used to investigate the dynamic changes of Ca2+ and cAMP in the ductal cells. The data were analysed statistically by t test. RESULTS:After an momently latent period, intercellular calcium increased rapidly, then began to decline. This period could last 5 minutes. After 3 minutes, cAMP showed a rapid decrease, and then increased slowly. Its concentration could restore 90 minutes later. CONCLUSION:EGF may regulate intercellular calcium and cAMP, which results in the proliferation of the ductal cells.

关 键 词：表皮生长因子 下颌下腺导管细胞 CA^2+ 钙 环磷酸腺苷 细胞增殖 作用机制 

分 类 号：R780.2[医药卫生—口腔医学]