长波紫外线照射对小鼠黑素瘤细胞的影响  

Effects of Ultraviolet A Irradiation on B16 Murine Melanoma Cells

作　　者：李玲[1] 周华[1] 宋伟民[1] 苏瑾[2] 

机构地区：[1]复旦大学公共卫生学院环境卫生学教研室,上海200032 [2]上海市疾病预防控制中心,上海200070

出　　处：《环境与职业医学》2004年第1期43-46,共4页Journal of Environmental and Occupational Medicine

摘　　要：[目的]研究不同剂量UVA照射对体外培养B_16小鼠黑素瘤细胞的形态、增殖率、黑素含量和酪氨酸酶活性的影响。[方法]选用B_16小鼠黑素瘤细胞 ,经不同剂量的UVA照射后 ,采用MTT法测定细胞的增殖 ,参照HideyaAndo等的方法测定酪氨酸酶活性和黑素含量。[结果]UVA照射后 ,黑素含量增加 (P<0.01) ,酪氨酸酶活性提高 (P<0.05) ,细胞增殖率上升 ,照射剂量在0.6、1.2、1.8J/cm2时 ,细胞增殖率的变化有显著性 (t=2.495、2.645、3.081,均为P<0.05)。[结论]随着照射剂量的增加 ,黑素含量增加 ,酪氨酸酶活性提高 ,细胞的增殖率增加 。Objective To investigate the effects of different doses of ultraviolet A(UVA)irradiation on the morphology,prolifˉeration,melanogenesis,tyrosinase activity of the cultured B16murine melanoma cells.[Methods] After B16melanoma cells were irradiated with UVA,MTTspectrometry was used to determine the proliferation of cells,Hideya Ando technique was applied to measure the synthesis of melanin,and the tyrosinase activity.[Results]It was found that a marked increase in melanin content(P<0.01)and tyrosinase activity(P<0.05)after irradiation with UVA.UVA could increase melanoma cell proliferation.[Conclusion]UVA could increase B16murine melanoma cell melanogenesis,tyrosinase activity and induce the proliferation in a dose-dependent manner.Howˉever,the effect of UVA irradiation on the induction of melanocyte proliferation was limited.

关 键 词：长波紫外线照射 小鼠 黑素瘤细胞 MTT法 细胞增殖 酪氨酸酶 细胞培养 

分 类 号：R735.9[医药卫生—肿瘤]