联立方程测定氯霉素霜中氯霉素含量  

Determination of Chloramphenicol in Chloramphenicol Cream by Simultaneous Equations

作　　者：陈元俊[1] 周金生[1] 卢玉兰[1] 翁开敏[1] 胡晓添[1] 连劲龙[1] 

机构地区：[1]中国人民解放军第180医院,泉州362000

出　　处：《海峡药学》2004年第1期49-51,共3页Strait Pharmaceutical Journal

摘　　要：目的 　建立氯霉素霜中氯霉素的含量测定方法。方法 　采用联立方程分光光度法 ,不经提取分离 ,将紫外相互干扰的主药氯霉素 (阳性 )与本制剂中的辅料 (阴性 )的最大吸收波长 2 5 8nm和 2 73 nm为测定波长 ,直接测定。 结果 　氯霉素的计算方程 :CL=5 9.660 8AK+ L2 73-3 6.862 1AK+ L2 58-0 .4174。其平均回收率为 10 0 .0 4% ,RSD=2 .40 %。结论 　本方法简便、快速 ,消除了各组分间紫外相互干扰 ,重现性好 ,结果满意。OBJECTIVE To establish a method that determined chloramphenicol in chloramphenicol cream.METHODS Simultaneous equations of spectophotometry was applied for directing determination of two compotents of chloramphenicol(positive)and adjuvant(negative)awithout separation.The detected wavelengths were 258nm and 273nm the maxmal absorbance of the two compotents.RESULTS The equations of chloramphenicol was:C L=59.6608A K+L 273 -36.8621A K+L 258 -0.4174。The corresponding average recovery was 100.04%for chloramphenicol whose RSD was2.40%。CONCLUSION The method is simple rapid and reproducible.The interference of the other compotents can be eliminated and the results are acceptable.

关 键 词：氯霉素霜 氯霉素 含量测定 联立方程分光光度法 皮肤科 

分 类 号：R986[医药卫生—药品] R927.2[医药卫生—药学]