双歧杆菌粘附素对脂多糖和H_2O_2调节肠上皮细胞增殖和凋亡的影响  

Effect of Bifidobacterial adhesin on lipopolysaccharide-and H_2O_2-induced proliferation and apoptosis of intestinal epithelial cells in vitro

作　　者：钟世顺[1] 张振书[1] 王继德[1] 赖卓胜[1] 潘令嘉[1] 

机构地区：[1]第一军医大学南方医院全军消化内科研究所,广东广州510515

出　　处：《第一军医大学学报》2004年第3期264-268,共5页Journal of First Military Medical University

基　　金：广东省自然科学基金资助(010621)~~

摘　　要：目的研究双歧杆菌分泌型粘附素对脂多糖(LPS)和H2O2体外调节肠上皮细胞增殖和凋亡的影响。方法用3H-TdR掺入法、流式细胞仪和荧光染色等方法观察肠上皮细胞增殖和凋亡的情况。结果100 μg/L LPS对细胞增殖和凋亡均有促进作用,200 μmol/L H2O2可抑制肠上皮细胞增殖,促进凋亡。丫啶橙荧光染色见细胞核染色质致密浓染或裂解成碎片。预先经双歧杆菌分泌型粘附素处理后,LPS组细胞增殖和凋亡率均显著下降,H2O2组细胞凋亡明显减少。结论在体外,双歧杆菌分泌型粘附素能抑制LPS和H2O2对肠上皮细胞的损害作用,维持肠上皮细胞增殖与凋亡的平衡。Objective To study the effect of Bifidobacterial adhesin on proliferation and apoptosis of intestinal epithelial cell induced by lypopolysaccharide (LPS) and H2O2 in vitro. Methods With 3H-TdR incorporation method, flow cytometry and fluorochrome staining, the proliferation and apoptosis of intestinal epithelial cells induced by LPS and H2O2 in vitro were studied. Results LPS at the dose of 100 μg/L effectively stimulated the proliferation and apoptosis of cells, whereas H2O2 at the dose of 200 μmol/L obviously restrained the proliferative ability while enhanced the apoptosis of the cells. Fluorochrome staining showed cell shrinkage, nuclear condensation and fragmentation under microscope. After treatment with Bifidobacterial adhesin, the cell proliferation and apoptosis decreased significantly in LPS group, and in H2O2 group, cell apoptosis was signifi- cantly decreased. Conclusions Bifidobacterial adhesin can protect intestinal epithelial cells from the damage by LPS and H2O2, and maintain the balance between the proliferation and apoptosis of the cells.

关 键 词：双歧杆菌粘附素 脂多糖 H2O2 调节 肠上皮细胞增殖 细胞凋亡 

分 类 号：R574[医药卫生—消化系统]