吲哚美辛对人结肠癌HCT116和SW480细胞的影响  

Effect of indomethacin on human colorectal cancer cells HCT116 and SW480

作　　者：王红梅[1] 张桂英[1] 

机构地区：[1]中南大学湘雅医院消化科,长沙410008

出　　处：《中南大学学报（医学版）》2004年第1期28-31,共4页Journal of Central South University ：Medical Science

基　　金：国家自然科学基金 (3 0 2 715 16)

摘　　要：目的 :探讨吲哚美辛对人结肠癌HCT116和SW4 80细胞的影响。方法 :体外培养细胞 ,采用MTT法分别检测吲哚美辛作用后两系细胞的生长情况 ,计算半抑浓度 (IC50 ) ;体内建立裸鼠皮下移植瘤模型 ,喂服吲哚美辛 3mg/ (kg·d)共 4周 ,隔日测瘤结节体积及鼠重 ,评价吲哚美辛的抑瘤效果。结果 :吲哚美辛作用于结肠癌HCT116和SW4 80细胞 4 8h后 ,细胞的生长均受到明显抑制 ,呈剂量依赖效应 ,IC50 分别为 (318.2± 12 .7) μmol/L和 (70 1.4± 2 9.5 ) μmol/L ;吲哚美辛显著减慢裸鼠皮下移植瘤的生长 ,用药 4周后抑瘤率达 4 4 .6 % ,未见明显毒性反应。结论 :吲哚美辛能抑制不表达环氧合酶的人结肠癌细胞 (HCT116和SW4 80 )生长 ,间接说明其抗癌作用不完全依赖环氧合酶途径 。Objective To explore the effect of indomethacin on COX non-expressing colon cancer cells (HCT116 and SW480).Methods We used MTT assay to assess the effect of indomethacin on the growth of the cultured cells. Transplanted tumor models were established with BALB/c nude mice and were treated with indomethacin at 3 mg/(kg·d) for 4 weeks. Tumor volume and mouse weight were recorded every other day. Histologic examination was performed.Results After 48-hour incubation with various concentrations of indomethacin, the growth of the two cell lines was significantly suppressed in a dose-dependent manner, with IC 50 being (318.2±12.7) μmol/L and (701.4±29.5) μmol/L, respectively. Indomethacin also effectively retarded the tumor growth within xenografts by 44.6% according to the tumor volume. No obvious toxicity was observed in these nude mice.Conclusion Indomethacin can inhibit the growth of COX-negative human colon cancer cells both in vitro and in vivo, which indirectly suggests that indomethacin exerts its anti-tumor effect at least partly independent of the cyclooxygenase inhibitory profile.

关 键 词：吲哚美辛 结肠癌 HCT116细胞 SW480细胞 MTT法 细胞培养 环氧合酶 非甾体抗炎药 

分 类 号：R735.35[医药卫生—肿瘤]