胎肝来源Sca-1^+细胞在体内分化为神经细胞研究  

Fetal liver - derived Sca- 1^+ cells can differentiate into both neurons and astrocytes in vivo

作　　者：刘革修[1] 张洹[1] 刘革修 张洹[1] 

机构地区：[1]暨南大学医学院血液病研究所，广东广州510632

出　　处：《中国病理生理杂志》2004年第3期354-358,共5页Chinese Journal of Pathophysiology

基　　金：Supported by Foundation of Science and Technology of Guangdong Province(No.99M01204G);Science and Technology of Guangzhou City(No.2001-2-037-01);Key Science Foundation of Guangdong Province(No.021195).

摘　　要：目的:为了了解胎肝干细胞是否具有向神经组织细胞分化的潜能。方法:PCR检测sry基因分析孕14.5d胚胎鼠性别;采用免疫磁珠法分离雄性胎鼠肝的Sca-1^+细胞,尾静脉注射Sca-1^+细胞(2.0×10~3个/小鼠)到致死剂量放射线照射的雌性小鼠。移植60、120、180 d后采用免疫组化和FISH双染色检测受体小鼠脑组织中供体来源细胞特性。结果:受体雌鼠脑组织内存在大最Y染色体阳性细胞。免疫组化分析显示,部分Y染色体阳性细胞表达神经组织特异标志,如神经元核特异蛋白(NeuN,Neuron-specific nuclei protein)、部分Y染色体阳性细胞表达星形胶质细胞特异标志,如胶质纤维酸性蛋白(GFAP,Glial fibrillary acidic protein)等。结论:移植的胎肝Sca-1^+细胞,含造血干细胞,能分化成神经细胞和星形胶质细胞。AIM: To determine whether Sca-1^+ cells from fetal liver can differentiate into neural cells. METHODS: The sex of 14.5-day-old murine fetuses was determined by PCR analysis of sry gene, and Sca-1^+ cells from male fetal liver were isolated with a magnetic cell sorting kit, 2×10~3 of which were then transplanted into lethally irradiated female mice. The donor cells and their characteristics in recipient brains were identified and detected by FISH and immunohistochemistry double-staining analysis at 60, 120, 180 days after transplantation. RESULTS: There existed many male cells in brains of female recipients, some of them express neuron-specific nuclear protein (NeuN), and some of them express the astrocyte-specific marker, i.e. glial fibrillary acidic protein (GFAP). CONCLUSION: Sca-1^+ cells from fetal liver, which contain hematopoietic stem cells, can differentiate into neuronal cells and astrocytes in the brains of adult mice.

关 键 词：分化 胎肝 神经元 SCA-1^+细胞 

分 类 号：R782[医药卫生—口腔医学]