CpG-ODN诱导白血病HL60细胞分化和凋亡的研究  

CpG-oligodeoxynucleotides induce differentiation and apoptosis in HL60 cells

作　　者：陈春燕[1] 贾继辉[2] 潘祥林[1] 张琦[1] 周亚滨[2] 阎世坤[2] 赵蔚明[2] 

机构地区：[1]山东大学第二医院血液肿瘤科,山东济南250033 [2]山东大学医学院微生物学教研室,山东济南250012

出　　处：《中国病理生理杂志》2004年第3期407-411,共5页Chinese Journal of Pathophysiology

基　　金：山东省医药卫生科研基金资助课题(1999CA2CAAB1)

摘　　要：目的:研究含CpG基序的寡核苷酸对白血病HL60细胞的作用。方法:设计合成含CpG基序的寡核苷酸(CpG-ODN)、不含CpG基序的寡核苷酸(nonCpG-ODN)和含甲基化CpC基序的寡核苷酸(ZpG-ODN)分别作用于白血病HL60细胞,MIT法测定HL60细胞抑制效应,硝基四氮唑蓝还原实验和细胞表面CD14分子表达状况分析HL60细胞诱导分化结果,流式细胞仪和透射电镜观察HL60细胞的凋亡现象,免疫组化检测HL60细胞后caspase 3、Bel-2和Bax的表达状况。结果:CpG-ODN对白血病HL60细胞有诱导分化和诱导凋亡作用,nonCPG-ODN和ZpG-ODN无此作用。结论:CpG-ODN可直接诱导白血病HL60细胞分化和凋亡,此为白血病免疫治疗提供了新的途径。AIM: To investigate the differentiative and apoptotic effect of CpG-oligodeoxynucleotides on HL60 cells and its mechanism. METHODS: After HL60 cells were exposed to synthetic CpG-oligodeoxynucleotides, non-CpG-oligodeoxynucleotides or ZpG-oligodeoxynucleotides for 72 hours, respectively, the inhibition of HL60 cells were detected using MTT method, NBT test was used and CD14 expression were determined. Apoptosis of HL60 cells were mensurated with flow cytometry and transmission electron microscope, and caspase 3, Bcl-2 and Bax expression of HL60 cells treated with oligodeoxynucletides were detected using immunohistochemistry. RESULTS: Treatment with CpG-oligodeoxynucleotides induced the differentiation and apoptosis in HL60 cells, but non-CpG-oligodeoxynu- cleotide and ZpG-oligodeoxynucleotide had no effect on HL60 cells. CONCLUSION: CpG-oligodeoxynucleotides can induce the differentiation and apoptosis in HL60 cells. It may provide a new approach for the immunological treatment of leukemia.

关 键 词：寡核苷酸类 HL60细胞 细胞凋亡 

分 类 号：R733.7[医药卫生—肿瘤]