苯那普利对自发性高血压大鼠心肌缺血-再灌注心功能、氧自由基和肌浆网Ca^(2+)-ATP酶的影响  

Effects of benazepril on cardiac function, free oxygen radicals, sarcoplasmic reticulum Ca^(2+)- ATPase following cardiac ischemia- reperfusion in spontaneously hypertensive rats

作　　者：王业松[1] 马虹[1] 陈健文[2] 胡苑[1] 蓝秀健[2] 邱烦华[2] 

机构地区：[1]中山大学附属第一医院心内科,广东广州510080 [2]中山大学附属第一医院生理实验科,广东广州510080

出　　处：《中国病理生理杂志》2004年第3期425-428,共4页Chinese Journal of Pathophysiology

基　　金：广州市科委基金(No.2002J1-CO181)

摘　　要：目的:探讨血管紧张素转换酶抑制剂(ACEI)对自发性高血压大鼠(SHR)心肌缺血-再灌注心功能、氧自由基和肌浆网Ca^(2+)-ATP酶的影响。方法:30只10周龄雌性SHR分为2组,SHR对照组(SHR)、SHR+B组(每日10mg/kg苯那普利);另15只同周龄、同性别Wistar大鼠作为对照组(Wistar)。治疗12周后,每组大鼠结扎左冠状动脉前降支30min,再灌注30min,观察血流动力学参数,左室心肌丙二醛(MDA)含量、超氧化物岐化酶(SOD)活性及肌浆网(SR)Ca^(2+)-ATP酶活性。结果:与Wistar组比较,SHR组血压、左心室重/体重较高,左心功能损害程度较重,心肌MDA含量较高,SOD活性和SRCa^(2+)-ATP酶活性较低;SHR+B组血压、左心室重/体重、左心功能损害程度,SR CA^(2+)-ATP酶活性无显著性差异,但心肌MDA含量较低,SOD活性较高。结论:苯那普利可逆转SHR左室肥厚,促进缺血-再灌注心肌SR Ca^(2+)-ATP酶活性的恢复和减少氧自由基损害,从而减轻缺血-再灌注心功能损伤。AIM: To investigate the effects of angiotensin converting enzyme inhibitor (ACEI), benazepril(B), on cardiac function, free oxygen radicals, sarcoplasmic reticulum(SR) Ca^(2+)-ATPase following ischemia-reper-fusion in sportaneously hypertensive rats (SHRs). METHODS: Thirty 10-week-old female SHRs were randomly assigned into two groups: group SHR was control; The animal in group SHR+B was given with 10 mg/kg of benazepril perday. Another 15 Wistar rats with the same age and sex were normal control (group Wistar). After 12 weeks of pretreatment, all rats in each group were subjected to 30 min of left anterior descending coronary artery occlusion and 30 min of reperfusion. Hemodynamic parameters, left heart-to-body weight ratio(LVW/BW), myocardial malondialdehyde (MDA) concentration, superoxide dismutase (SOD) activity, and SR Ca^(2+)-ATPase activity were measured. RESULTS: Compared to group Wistar, the rats in group SHR had higher blood pressure, LVW/BW and myocardial MDA concentration, more serious left cardiac function injury and lower myocardial SOD activity and SR Ca^(2+)-ATPase activity; group SHR+B had lower myocardial MDA concentration, higher myocardial SOD activity, but no difference in blood pressure, LVW/BW, the degree of left cardiac function injury and myocardial SR Ca^(2+)-ATPase activity. CONCLUSION: Benazepril can attenuate ischemia-reperfusion-induced cardiac function injury by regression of left ventricular hypertrophy (LVH), improving SR Ca^(2+)-ATPase activity and decreasing oxygen free radicals injury in SHRs.

关 键 词：苯那普利 缺血-再灌注 心功能 氧自由基 CA^2+-ATP酶 

分 类 号：R363[医药卫生—病理学]