大鼠肺纤维化形成中肺巨噬细胞增殖和凋亡的变化  

Changes in proliferation and apoptosis of macrophages in the development of pulmonary fibrosis of rats

作　　者：陈晓玲[1] 黄善生[1] 李文斌[1] 艾洁[1] 

机构地区：[1]河北医科大学基础医学研究所病理生理研究室,河北石家庄050017

出　　处：《中国病理生理杂志》2004年第3期433-436,共4页Chinese Journal of Pathophysiology

基　　金：河北省教育厅博士科研基金(B2002215)

摘　　要：目的:观察肺纤维化形成过程中,肺泡巨噬细胞数量、增殖和凋亡的变化。方法:气管内滴注平阳霉素(BLMA_5)(5mg/kg),观察注后14d和30d组大鼠支气管肺泡灌洗液(BALF)中肺泡巨噬细胞数量、增殖和凋亡的变化以及细胞的MTT活力。结果:(1)BLMA_5 14d组和30d组大鼠BALF中巨噬细胞数分别多于sham 14d和30d组,(分别P<0.01,P<0.05);但BLMA_5 30d组的细胞数明显少于BLMA_5 14d组(P<0.05);(2)BLMA_5 14d组巨噬细胞增殖指数高于sham 14d组(P<0.05),而BLMA_5 30d组增殖指数低于sham 30d组(P<0.05);(3)BLMA_5 14d和30d组凋亡细胞数分别多于sham 14d和30d组(P<0.01),但BLMA_5 14d组少于BLMA_5 30d组(P<0.05);(4)BLMA_5 14d和30d组大鼠BALF中巨噬细胞数MTT活力分别高于sham 14d和30d组,分别P<0.01,P<0.05。结论:在肺纤维化形成过程中,肺巨噬细胞增殖能力先增强后减弱;而肺巨噬细胞凋亡始终增加,上述变化是导致肺巨噬细胞数量和功能变化的因素之一。AIM: To observe the changes in proliferation and apoptosis of macrophages in the development of pulmonary fibrosis in rats. METHODS: The number of macrophages, apoptotic cells, the proliferation index (PI) and MTT activity of macrophages were assayed on the day 14 and the day 30 after intratracheal adminstration BLMA_5 in rats.RESULTS: (1) The number of alveolar macrophages was increased in BLMA_5 14 d group and in BLMA_5 30 d group, compared with sham 14 d group and sham 30 d group, respectively. The number of macrophages in BLMA_5 14 d group was higher than that in BLMA_5 30 d group. (2) The PI of macrophages increased in BLMA_5 14 d group, and decreased in BLMA_5 30 d group. (3) The number of apoptotic cells increased both in BLMA_5 14 d group and BLMA_5 30 d group.The number of apoptotic cells in BLMA_5 14 d group was lower than that in BLMA_5 30 d group. (4) The MTT activity of macrophages was higher in BLMA_5 14 d group and in BLMA_5 30 d group than that in sham 14 d group and sham 30 d group, respectively. CONCLUSIONS: The ability of proliferation increased at first, and then decreased, but the apoptosis of macrophages increased all the time, in the development of pulmonary fibrosis. This might be partly contributed to the changes of the number and function of macrophages in lung.

关 键 词：肺纤维化 巨噬细胞 肺 大鼠 细胞凋亡 

分 类 号：R363[医药卫生—病理学]