胱蛋白酶抑制剂相关的附睾精子发生基因在不同发育阶段小鼠睾丸和附睾中的表达  

Expression of the Cystatin-related Epididymal Spermatogenic Gene in Mouse Testes and Epididymis at Different Postnatal Stages

作　　者：袁青[1] 徐晨[1] 张小瑾[1] 陈海珍[1] 王一飞[1] 

机构地区：[1]上海第二医科大学组织胚胎学教研室,上海200025

出　　处：《中华男科学杂志》2004年第3期168-171,共4页National Journal of Andrology

基　　金：国家自然科学基金 (3 0 0 70 3 91);973基础研究基金(G19990 5 5 90 1);上海市人口与计划生育委员会科技发展基金重点项目(0 3JG0 5 0 0 9)资助

摘　　要：目的 :研究胱蛋白酶抑制剂相关的附睾精子发生 (Cres)基因在生后不同发育阶段小鼠睾丸及附睾中的表达规律。　方法 :采用半定量RT PCR方法检测CresmRNA在生后 14、2 0、2 2、2 8、35、4 9、70、4 0 0d小鼠睾丸及附睾中的表达变化。　结果 :Cres基因在 14d小鼠睾丸和附睾中呈低水平表达 ,随着小鼠的生长发育 ,CresmRNA的表达量逐渐升高。在 70d小鼠睾丸和 4 0 0d小鼠附睾中 ,CresmRNA的表达量达到峰值。　结论 :Cres基因在生后不同发育阶段小鼠睾丸及附睾中呈现明显的规律性表达 ,可能参与精子发生、成熟过程的调控。Objective: To investigate the expression pattern of the cystatin related epididymal spermatogenic( Cres ) gene in mouse testes and epididymis during postnatal development. Methods: Semi quantitative RT PCR was used to detect the Cres mRNA level in postnatal mouse testes and epididymis on day 14, 20, 22, 28, 35, 49, 70 and 400. Results: Low level Cres mRNA was detected on day 14 in both the testes and the epididymis. The expression of the Cres gene increased gradually with the development of the mouse, and it reached the peak on day 70 in the testes and on day 400 in the epididymis. Conclusion: The time dependent expression pattern of the Cres gene in postnatal mouse testes and epididymis suggested that the Cres gene might be involved in the regulation of spermatogenesis and sperm maturation.

关 键 词：Cres基因 发育 睾丸 附睾 胱蛋白酶抑制剂 精子发生基因 

分 类 号：R33[医药卫生—人体生理学]