微卫星不稳定在胃癌中的发生及其与胃癌临床病理特点的关系  

Frequency of microsatellite instability in gastric cancer and its relationship with clinicopathological features of gastric cancer

作　　者：邵华[1] 刘顺英[1] 龚永驰[1] 

机构地区：[1]东南大学附属中大医院消化内科,江苏南京210009

出　　处：《东南大学学报（医学版）》2004年第2期96-99,共4页Journal of Southeast University(Medical Science Edition)

摘　　要：目的 :研究微卫星DNA不稳定性 (MSI)在胃癌发生中的作用及其与胃癌临床病理之间的关系。方法 :采用PCR、PCR 变性聚丙烯酰胺凝胶及硝酸银染色方法检测 3 0例胃癌组织的MSI。结果 :胃癌MSI阳性率为 3 6.67% (11/3 0 ) ,MSI与胃癌患者的年龄、性别、胃癌分期、淋巴结转移及组织分型无关 (P >0 .0 5 ) ,与胃癌的分化程度和发生部位有关 :高 中分化胃癌high levelMSI(MSI H)检出率 (4 7.0 6% ,8/17)显著高于低分化胃癌 (7.69% ,1/13 ,P <0 .0 5 ) ;胃窦胃癌MSI H阳性率(4 7.3 7% ,9/19)显著高于胃体及胃底胃癌 (0 ,0 /11,P =0 .0 1)。结论 :MSI可能在胃癌的发生中起一定作用 。Objective To investigate the role of microsatellite instability(MSI) in carcinogenesis of gastric cancer(GC)and its relationship with clinicopathological features of GC.Methods MSI was examined in 30 surgically resected GC specimens using PCR,PCR denatured polyacrylamide gel based methods.Results MSI was detected in 36.67%(11/30) of GC.No significant correlation was found between MSI status and age,sex,clinical stage,lymph node metastasis and histological types of GC( P >0.05),but there seems a correlation between MSI and differentiation or location of GC.The incidence of MSI?H(high level MSI) in well or moderately differentiated GC(8/17, 47.06% ) was significantly higher than that in poorly differentiated GC(1/13,7.69%)( P <0.05),and MSI?H occurred more frequently in GC located in antrum(9/19,47.37%)than that in body and fundus(0/11,0%)( P = 0.01 ).Conclusion MSI may play a certain role in gastric carcinogenesis,and the relationship between MSI and clinicopathological features of GC needs further investigation.

关 键 词：胃癌 微卫星不稳定 临床病理学 聚合酶链反应 变性聚丙烯酰胺凝胶 硝酸银染色 

分 类 号：R735.2[医药卫生—肿瘤]