复合抗氧化剂对2型糖尿病患者糖负荷时炎性细胞因子表达的影响  

Effects of complex antioxidant on the expression of inflammatory cellular factor under glucose load in patients with type 2 diabetes mellitus

作　　者：张帆[1] 朱翠凤[2] 戴雅丽[1] 蓝薇[1] 徐晓燕[1] 苏宜香[3] 郭松超[4] 

机构地区：[1]深圳市北京大学深圳医院内分泌科,广东省深圳市518036 [2]深圳市北京大学深圳医院营养科,广东省深圳市518036 [3]中山大学公共卫生学院营养系,广东省广州市510080 [4]广西医科大学公共卫生学院营养系,广西壮族自治区南宁市530021

出　　处：《中国临床康复》2004年第9期1693-1695,共3页Chinese Journal of Clinical Rehabilitation

基　　金：广西壮族自治区科技厅科技攻关项目资助(桂科攻0322025-6)~~

摘　　要：目的:探讨不同剂量的大豆异黄酮、维生素C和维生素E复合抗氧化剂协同作用对2型糖尿病患者糖负荷时血管内皮炎性细胞因子表达的影响。 方法:将120例符合条件的2型糖尿病患者按性别和体质量指数分层后随机分为4组(分别为糖尿病对照组、低剂量组、中剂量组、高剂量组),进行口服葡萄糖耐量试验(OGTT)时分别给予不同剂量的大豆异黄酮、维生素C和维生素E的混合干预物或安慰剂并与正常人(正常对照组)比较,观察各组在OGTF时0,1,2,4,6h血清中肿瘤坏死因子α(TNF-α)和白细胞介素6(IL-6)的变化。 结果:4个2型糖尿病组空腹时TNF-α水平显著高于正常对照组(F=8.76,P<0.01)。OGTT中糖尿病对照组IL-6水平于餐后1h[(54.03±99.26)μg/L]达峰值,1,2 h均显著高于空腹[(54.65±104.70)μg/L](F=9.12,P<0.01;F=3.56,P<0.05);TNF-α水平于餐后2h[(67.26±95.05)μg/L]达峰值,2,4h均显著高于空腹(F=10.23,9.56,P<0.01)、1h(F=3.45,3.26,P<0.05)和6h(F=11.36,10.05,P<0.01)。低剂量组波动趋势同糖尿病对照组,IL-6水平餐后1h达高峰显著高于空腹(F=4.13,P<0.05);TNF-α水平餐后于餐后2h达峰值,2,4h均显著高于0 h(F=8.97,8.56,P<0.01)、1h(F=3.12,3.01,P<0.05)和6 h(F=9.01,8.87,P<0.01)。4h开始下降,至6 h时回落至空腹水平。AIM: To investigate the co-effect of different dosages of antioxidant (isoflavone, vitamin C and E) on the expression of endothelium inflammatory cellular factor under glucose load in patients with type 2 diabetes mellitus. METHODS: Totally 120 qualified patients with type 2 diabetes were divided into 4 groups after delamination according to sex and body mass index: diabetic control group, low, middle and high dose groups, all the patients were treated by different doses mixture of isoflavone. VC and VE or placebo while oral glucose tolerance test(OGTT), and were compared with the normal subjects 1 normal control group). The changes of tumor necrosis factor-alpha (TNF-α) and interleukin 6 (IL-6) in serum were observed when fasting, 1, 2, 4 and 6 h during OGTT respectively in each group. RESULTS: At fasting level. TNF-a in the four type 2 diabetic groups were significantly higher than that in the control group ( F = 8. 76, P < 0. 01) . After OGTT. IL-6 level reached the peak at 1 h in the diabetic control group, those of 1 and 2 h were both higher than fasting one ( F = 9.12. P < 0. 01; F = 3. 56, P < 0. 05); TNF-α reached the peak at 2 h. those of 2 and 4 h were all significandy higher than 0 h( F = 10. 23. 9. 56, P < 0. 01). 1 h (F = 3.45, 3.26. P < 0. 05)and 6 h( F = 11. 36. 10.05, P < 0.01). The fluctuating tendency in the low dose group was the same as the diabetic control group: IL-6 level reached the peak at 1 h[ 1.54. 03 ±99. 26)μg/L], significantly higher than fasting one [ (54. 65 ± 104. 70) μg/L] ( F = 4. 13. P < 0. 05); TNF-α reached the peak [(67. 26 ± 95. 05) μg/L] at 2 h, those of 2 and 4 h [ (65. 56 ± 99. 62) μg/L] were all significantly higher than fasting( F = 8. 97, 8.56, P < 0. 01), 1 h( F = 3. 12, 3.01, P < 0. 05) and 6 h( F = 9.01, 8.87. P < 0. 01) ( F= 10. 23, 9. 56. P< 0. 01), it began to decrease at 4 h, and reduced to the fasting level at 6 h. The postprandial IL-6 and TNF-α levels in the middle and high dose groups were not as high as those in the normal control group, diabet

关 键 词：复合抗氧化剂 2型糖尿病 糖负荷 炎性细胞因子 大豆异黄酮 维生素C 维生素E 

分 类 号：R587.1[医药卫生—内分泌] R977[医药卫生—内科学]