Simultaneous Determination of Five Components Including Acetaminophen by Reversed-phase High Performance Liquid Chromatography  

作　　者：张立庆 

机构地区：[1]Zhejiang University of Science and Technology

出　　处：《Journal of Wuhan University of Technology(Materials Science)》2004年第1期34-35,共2页武汉理工大学学报（材料科学英文版）

基　　金：FinanciallysupportedtheScienceFoundationofAnalysisTestofZhejiangProvince (No.0 30 84 )

摘　　要：High performance liquid chromatography with a C18 reverse-phase column was used to separate the five components in cough syrup,including acetaminophen,p-aminophenol,caffeine,chlorphenamine maleate and guaifenesin.The mobile phase consists of 15wt% acetonitrile,0.004mol/L sodium heptyl sulfonate, 0.03mole/L potassium di-hydrogen phosphate and triethylamine (volume ratio 13∶40∶44∶3),the pH of which is adjusted to 3.0 by phosphoric acid.The contents of the five components are analyzed on an ultraviolet spectrophotometer at 254nm,with a flow rate of 0.4mL/min.The results show that the calibration curves are linear in a certain range.The average recovery of five components is between 96.31% and 102.3%.High performance liquid chromatography with a C18 reverse-phase column was used to separate the five components in cough syrup,including acetaminophen,p-aminophenol,caffeine,chlorphenamine maleate and guaifenesin.The mobile phase consists of 15wt% acetonitrile,0.004mol/L sodium heptyl sulfonate, 0.03mole/L potassium di-hydrogen phosphate and triethylamine (volume ratio 13∶40∶44∶3),the pH of which is adjusted to 3.0 by phosphoric acid.The contents of the five components are analyzed on an ultraviolet spectrophotometer at 254nm,with a flow rate of 0.4mL/min.The results show that the calibration curves are linear in a certain range.The average recovery of five components is between 96.31% and 102.3%.

关 键 词：organic compounds preparation and determination CHROMATOGRAPHY 

分 类 号：O657.3[理学—分析化学]