鼠胚胎癌细胞的诱导分化和间隙连接形成  

Induced differentiation and gap junction formation of murine embryonal carcinoma cells

作　　者：孙君洁[1] M.W.麦克柏尼 

机构地区：[1]昆明医学院 [2]渥太华大学

出　　处：《中国病理生理杂志》1992年第3期277-280,共4页Chinese Journal of Pathophysiology

摘　　要：在原有研究的基础上,本文进一步证实鼠胚胎癌细胞系P_(19)的突变型αaS1可以被维生素A酸(RA)诱导分化,但对DMSO不敏感。在被一定浓度的RA诱导分化时,αaS1细胞同样出现了增生和分化间呈动态平衡的平稳阶段。与其他突变型相比,αaS1,细胞形成间隙连接的能力较差,这表明EC细胞突变型的某一种间隙连接型可能会出现缺陷。P_(19)和RAJH_6的混合细胞集合物在含RA的培养液中,只有前者被不断诱导分化,但经6-TG处理后,存活的RAJH_6细胞的分数基本平稳。此项结果提示,正在分化的EC细胞和未分化的EC细胞之间可形成代谢偶联。我们的工作有助于进一步了解细胞分化、先天畸形和肿瘤逆转。Based on the work we have previously done, the experiments presented here indicate that αaS1, a mutant of the P19 line of routine embryonal carcinoma (EC) cells, can be induced to differentiate by retinoic acid (RA), but is not sensitive to DMSO. Also there is a dynamic equilibrium achieved by a balance between the processes of αaS1 cell proliferation and differentiation when exposed to certain RA concentrations. The αaS1 cell has a lower ability for forming gap junctions as compared with the other mutants, showing that EC cell mutants could be defective in the development of a certain gap junction type. In the RA-treated mixed aggregates of P19 and RAJH6, only P19 cells differentiated continuously but the surviving fraction of RAJH6 cells in the presence of 6-TG kept unchanging. The result suggests that the undifferentiated and differentiating EC cells can be metabolically coupled by gap junctions. Our work may further the understanding of cell differentiation, congenital abnormality and tumor reversion.

关 键 词：胚胎癌 小鼠 细胞分化 间隙连接 

分 类 号：R730.2[医药卫生—肿瘤]