亚硒酸钠对交链孢酚诱发DNA单链断裂和脂质过氧化的影响  

The effects of sodium selenite on the AOH-induced DNA single strand breaks and lipid peroxidation

作　　者：贾庆梅[1] 刘桂亭[1] 钱玉珍[1] 宫亚欧[2] 

机构地区：[1]河南医科大学病理生理学教研室 [2]河南医科大学食管癌研究室

出　　处：《中国病理生理杂志》1992年第4期371-375,共5页Chinese Journal of Pathophysiology

摘　　要：本文采用碱性洗脱-DNA荧光分析法及硫代巴比妥酸反应法,研究了亚硒酸钠(Na_2SeO_3)对交链孢酚(alternariol,AOH)诱发DNA单链断裂和脂质过氧化的影响。结果表明,在人胚食管上皮组织或人胚肺2BS细胞株接触AOH前4小时,在培养基内加10^(-5)或10^(-6)M Na_2SeO_3能显著抑制AOH诱导的DNA单链断裂和脂质过氧化反应;Na_2SeO_3与AOH同时处理对AOH的上述诱变性作用无显著抑制作用,10^(-4)MNa_2ScO_3可诱发人胚肺2BS细胞株DNA单链断裂。结果提示硒能抑制AOH的诱变性,但高浓度硒有毒性作用。The alkaline elution and flurometric DNA assay for the mesurement of the DNA single-strand breaks (SSB) and the thiobarbituric, acid test for the mesurement of molondialdehyde (MDA) were adopted in this article to study the effects of sodium selenite (Na_2SeO_3) on the mutagenicity of AOH. The results showed that pretreating the cells or epithelia with 10^(-5) or 10^(-6)M Na_2SeO_3 for 4 hours prior to exposure to AOH could inhibit the AOH-induced DNA SSB and the content of MDA which was the terminal pro duct of the lipid peroxidation (comparing with the control group, P<0.01 and P<0.001). Treating the cells or epithlia with 10^(-5)～10^(-7) M Na_2SeO_3 and AOH simutaneously had no above inhibition effects.The results also showed that treating the human fetal lung 2BS cells with 10^(-4) M Na_2SeO_3 for 3 hours could induce the DNA SSB (compare with the control, P<0.01). But 10^(-4)～10^(-7)M Na_2SeO_3 had no significant effects on the content of MDA in the epithelium of the human fetal esophagus. The results suggest that selenium could inhibit the mutagenicity of AOH, however high dosage selenium is toxic and mutagenic.

关 键 词：硒 食管肿瘤 链格孢霉属 

分 类 号：R730.23[医药卫生—肿瘤]