感染小鼠白血病病毒的L783V/3T3细胞系的建立及鉴定  

Establishment and identification of L783V/3T3 cell line infected with leukemia viruses

作　　者：吕彪[1] 季文琴[1] 程立[1] 黄春妹 宋后燕[2] 

机构地区：[1]上海医科大学病理生理教研室 [2]上海医科大学分子遗传教研室

出　　处：《中国病理生理杂志》1992年第6期569-572,共4页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金

摘　　要：L783是上海医科大学病理生理教研室七十年代建立的一株小鼠可移植性淋巴细胞型白血病。用L783发病小鼠的血液、脾脏等组织的无细胞提取液,分别感染NIH-3T3培养细胞,建立了L783小鼠白血病病毒感染的NIH-3T3细胞系(L783V/3T3)。实验结果表明:L783V/3T3细胞的XC合胞形成试验阳性,电镜下可观察到典型的A型和C型病毒颗粒。制备L783V/3T3细胞无细胞提取液,接种新生昆明种乳鼠,能诱发淋巴细胞型白血病。L783 murine lymphocytic leukemia was established in 1978 by Dept. of Pathophysiology of Shanghai Medical University. L783V/3T3 cell line was established by infecting NIH-3T3 cells with the cell-free extracts of blood, spleen respectively of L783 mice. The plaque assay of L783V/3T3 cells was positive, typical A-type and C-type virus particles could be observed under electronmicroscope. Lymphocytic leukemia could be induced by inoculation of cell-free extracts from L783V/3T3 cells into Kunming strain newborn mice.

关 键 词：白血病病毒 白血病 淋巴细胞性 鼠 

分 类 号：R733.730.3[医药卫生—肿瘤]