低剪应力作用下内皮细胞的基因差异表达  

DNA Microarray Reveals Changes in Gene Expression of Endothelial Cells under Shear Stress

作　　者：成敏[1] 张文胜[2] 陈槐卿[1] 吴文超[1] 黄华[1] 

机构地区：[1]四川大学华西医学中心生物医学工程研究室,成都610041 [2]四川大学华西医院麻醉科,成都610041

出　　处：《生物医学工程学杂志》2004年第2期188-192,共5页Journal of Biomedical Engineering

基　　金：国家自然科学基金资助项目 (3 983 0 110 );CMB基金部分资助

摘　　要：用含 4 0 96点的 c DNA芯片高通量、大范围、高效率地研究人脐静脉内皮细胞受剪应力 (4.2 0 dyne/cm2 ,2 h)作用后基因的表达情况 ,以期了解低剪应力短时间作用对脐静脉内皮细胞基因表达的影响。结果显示 :低剪应力作用内皮细胞后与未行剪应力处理的对照组比较 ,共有 10 8条基因 (5 3条基因表达增加 ,5 5条基因表达下降 )出现差异表达 ,约占芯片上基因总数的 0 .0 2 6 %。其中抑癌基因、与脂质代谢的有关基因明显上调 ,而与应激反应、细胞代谢的有关基因的表达则明显降低。本实验为寻找内皮细胞剪应力诱导表达的新基因提供了新的思路。cDNA microarray technology is used as a powerful tool for rapid, comprehensive, and quantitative analysis of gene profiles of cultured human umbilical vein endothelial cells(HUVECs) in the normal static group and the shear stressed (4.20 dyne/cm 2, 2 h) group. The total RNA from normal static cultured HUVECs was labeled by Cy3 dCTP, and total RNA of HUVECs from the paired shear stressed experiment was labeled by Cy5 dCTP. The expression ratios reported are the average from the two separate experiments. After bioinformatics analysis, we identified a total of 108 genes (approximately 0.026 %) revealing differential expression. Of these 53 genes expressions were up regulated, the most enhanced ones being human homolog of yeast IPP isomerase, human low density lipoprotein receptor gene, Squalene epoxidase gene,7 dehydrocholesterol reductase, and 55 were down regulated, the most decreased ones being heat shock 70kD protein 1, TCB gene encoding cytosolic thyroid hormone binding protein in HUVECs exposed to low shear stress. These results indicate that the cDNA microarray technique is effective in screening the differentially expressed genes in endothelial cells induced by various experimental conditions and the data may serve as stimuli to further researches.

关 键 词：低剪应力作用 内皮细胞 基因差异 基因表达 基因芯片 

分 类 号：R318.01[医药卫生—生物医学工程]