类金刚石薄膜成分变化对蛋白吸附的影响  

Effects of the Variation in Components of DLC on Protein Adsorption

作　　者：李伯刚[1] 康云清[1] 尹光福[1] 郑昌琼[1] 

机构地区：[1]四川大学材料科学与工程学院,成都610065

出　　处：《生物医学工程学杂志》2004年第2期193-195,共3页Journal of Biomedical Engineering

基　　金：国家自然科学基金 (3 9870 2 3 2 );教育部博士学科点专项科研基金(2 0 0 0 610 0 4)资助项目

摘　　要：采用全方位离子注入离子束增强沉积工艺制备类金刚石薄膜 (DL C) ,再将 DL C进行有氧和低压氩气保护条件下的热处理 ,分别得到富石墨相 DL C和富金刚石相 DL C。通过 X光电子能谱分析了三种 DL C的碳相组成 ,用放射性同位素 1 2 5I标记法 ,测定了恒温条件下人血白蛋白 (HSA)、纤维蛋白原 (HFG)和免疫球蛋白 (Ig G)在 3种 DL C材料表面的吸附量。结果 ,经两种不同的热处理工艺 ,DL C中石墨相和金刚石相分别增加了一倍左右 ;随着石墨、金刚石等杂质相的增加 ,DL C对 HSA的吸附能力下降 ,对 HFG和 Ig G的吸附能力显著提高 ,同时蛋白吸附特性也由原来对 3种蛋白的非特异性吸附 ,转变为对 HFG和 Ig G的优先吸附。这一结果表明 ,石墨和金刚石等杂质相将严重影响 DL C的蛋白吸附性能 ,并进而对 DL C的血液相容性产生负面影响。Diamond like carbon(DLC) was prepared by means of plasma source ion implantation ion beam enhanced deposition. Through the heat treatment upon DLC in air and in depressed Ar gas, the DLC rich in graphite,DLC rich in diamond, and other kinds of DLC used in the study were obtained respectively. For the three kinds of DLC, the components of carbonaceous phase were analysed by X ray photoelectron spectroscopy (XPS), the adsorptive amounts of human serum albumin(HSA), human serum fibrinogen(HFG) and human serum immunoglobin(IgG) on their surfaces in the condition of constant temperature were determined by radio isotope 125 I labelling method. Results showed the graphite phase and diamond phase in DLC increased by two times or so respectively after the aforementioned different heat treatment. In pace with the increase of these foreign phases, the adsorptive amounts of HFG and IgG on DLC greatly increase but the adsorptive amounts of HSA on DLC decrease; furthermore, there is a change from non selective adsorption of three human serum proteins into selective adsorptions of HFG and IgG prior to HSA. These results indicate that the foreign phases in DLC such as graphite, diamond, C H phase and C O phase have a great effect on protein adsorption on DLC and hence a negative effect on the hemocompatibility of DLC. The mechanisms for the increase of graphite phase and diamond phase in the process of heat treatment were also discussed in this paper.

关 键 词：类金刚石薄膜 蛋白吸附 血液相容性 DLC 免疫球蛋白 IGG 

分 类 号：R318.08[医药卫生—生物医学工程]