胶质瘤多药耐药细胞株大鼠动物模型的建立及其生物学性质  

Rat Model for the Multidrug Resistant Glioma Cell Line

作　　者：张健[1] 张庆林[1] 王成伟[1] 于骀飞[1] 郭华[1] 孔建新[1] 陶荣杰[1] 刘福生[1] 

机构地区：[1]山东大学第二医院神经外科,济南250033

出　　处：《中国医学科学院学报》2004年第1期43-46,共4页Acta Academiae Medicinae Sinicae

基　　金：国家自然科学基金(30070272)资助~~

摘　　要：目的建立神经胶质瘤多药耐药细胞株C6/adr及其原始细胞株C6种植在S-D大鼠脑内的动物模型,对照研究其生物学性质。方法体外培养神经胶质瘤多药耐药细胞株C6/adr及其原始细胞株C6,在磁共振连续动态监测下,进行大鼠脑内接种,于接种当天、第3天以及接种后每周进行磁共振随访检查,并对同期大鼠进行组织活检,观察接种后肿瘤大小变化、荷瘤大鼠的生存期。结果体外培养的神经胶质瘤多药耐药细胞株接种大鼠4周以后出现明显的颅内压增高症状,磁共振阳性结果远早于症状的出现,能更准确地动态显示肿瘤在脑内的生长情况,接种后同期病理结果证实了磁共振影像学表现。C6和C6/adr细胞大鼠动物模型的生长特性相似,两者接种后同期的肿瘤大小、生存期无显著差异(P>0.05)。结论应用磁共振对大鼠动物模型进行动态监测,结合同期的病理切片,能够全面了解活体肿瘤的生长情况,并可确立大鼠脑内肿瘤生长的早、中、晚分期,为体内实验的进一步深入研究提供重要的工具和必要的基础。Objective To evaluate the animal model of the multidrug resistant glioma cell line C6/adr for further in vivo studies.Methods The rat glioma cells C6and multidrug resistance cells C6/adr were cultured in vitro and implanted into the brain of S-D rats.After implantation,all these animals were examined continually with magnetic resonance imaging(MRI)and histological examination.The growth procedure of intracranial implanted glioma and the survival span of the animal model were evaluated.The statistical analysis was made between the survival data of the two cell lines.Results The symptoms of intracranial hypertension did not occur until4weeks after inoculation.The MRI findings of the implanted glioma in the rat brain were much earlier than the abnormal behavior observed.Pathological results after inoculation demonstrated the MRI findings.The two cell lines had similiar growth characteristics and no significant differences in survival times.Conclusion These results suggest that by means of MRI and histology the growth procedure of the implanted glioma in vivo be successfully observed.All these data will proved to be a useful basis for study of glioma in vivo.

关 键 词：胶质瘤 多药耐药 动物模型 磁共振 

分 类 号：R739.41[医药卫生—肿瘤]