促血管生成素-2对胃癌血管生成的双向效应  

Dual effect of angiopoietin-2 on angiogenesis in gastric cancer

作　　者：陈林莺[1] 张声[1] 林建银[2] 黄培生[3] 

机构地区：[1]福建医科大学附属第一医院病理科,福州350005 [2]福建医科大学分子医学教研室 [3]福建医科大学病理学系

出　　处：《中华肿瘤杂志》2004年第3期161-164,共4页Chinese Journal of Oncology

基　　金：福建省自然科学基金资助项目 (C0 110 0 13 )

摘　　要：目的　探讨促血管生成素 2 (Ang 2 )在胃癌血管生成中的作用。方法　运用RT PCR和S P免疫组化方法检测Ang 2mRNA、血管内皮生长因子 (VEGF)、CD34蛋白在 36例胃癌及其相应癌旁胃黏膜组织中的表达。结果　胃癌组织及其相应癌旁胃黏膜组织均见有Ang 2mRNA阳性表达 ,胃癌组织Ang 2mRNA的总体表达水平与微血管密度 (MVD)未见明显相关。胃癌组织Ang 2mRNA表达水平低于癌旁胃黏膜组织者 2 7例 ,其癌组织中 ,Ang 2mRNA的表达水平与MVD呈正相关 (r=0 .4 11,P <0 .0 5 ) ;同时 ,VEGF阳性表达者的MVD(45 .4 5± 10 .30 )明显高于VEGF阴性染色者 (30 .15± 8.6 9,P <0 .0 5 ) ,即在Ang 2表达上调的情况下VEGF促进血管生成。胃癌组织Ang 2mRNA表达水平高于癌旁组织者 9例 ,其癌组织中Ang 2mRNA的表达水平与肿瘤组织的MVD呈负相关 (r =- 0 .75 8,P <0 .0 5 ) ,VEGF的阳性表达者与阴性染色者间 ,MVD差异无显著性 ,即Ang 2抑制血管生成与VEGF的表达无相关性。结论　Ang 2对胃癌血管生成具有双向调节作用。ObjectiveTo explore the effect of Ang-2 on angiogenesis in gastric cancer. MethodsThe expression of Ang-2 mRNA was analyzed by RT-PCR and the expression of VEGF and CD34 was detected by immunohistochemistry in 36 cases of gastric cancer tissues and their paired adjacent gastric mucosa. ResultsThe expression of Ang-2 mRNA was found both in gastric cancer and their paired adjacent gastric mucosa; the correlationship between the general expression levels of Ang-2 mRNA and microvessel density (MVD) in gastric cancer tissues was not found. However, in 27 cases whose Ang-2 mRNA expression levels in cancer tissues were lower than those in adjacent gastric mucosa. A significant positive correlation between the expression level of Ang-2 mRNA and MVD in the tumor tissues was found( r =0.411, P <0.05). In these 27 cases, the MVD in the gastric cancer tissues with positive VEGF expression(45.45±10.30) was higher than that with negative VEGF expression(30.15±8.69, P <0.05), whereas in the other 9 cases whose expression levels of Ang-2 mRNA in cancer tissues were higher than those in adjacent gastric mucosa, a significant negative correlation between expression level of Ang-2 mRNA and MVD in the tumor tissues( r =-0.758, P <0.05), but without correlation between the MVD and VEGF. ConclusionUnder the conditions that the expression of Ang-2 mRNA in cancer tissues was lower than that in adjacent gastric mucosa, VEGF could promote the sprouting of new vessels along with Ang-2 upregulation. But under the conditions that the expression of Ang-2 mRNA in cancer tissues was higher than that in adjacent gastric mucosa, Ang-2 inhibited angiogenesis. Angiopoietin-2 may play a dual effect on angiogenesis in gastric cancer.

关 键 词：促血管生成素-2 胃癌 血管生成 双向效应 RT-PCR S-P免疫组化法 肿瘤侵袭 

分 类 号：R735.2[医药卫生—肿瘤]