酒石酸锑钾诱导人胃癌细胞凋亡  

Potassium antimonyl tartrate induces apoptosis in human gastric carcinoma cells

作　　者：郭光云[1] 徐少勇[1] 邓长生[2] 

机构地区：[1]郧阳医学院附属人民医院消化内科,湖北省十堰市442000 [2]武汉大学中南医院消化内科,湖北省武汉市430071

出　　处：《世界华人消化杂志》2004年第3期520-522,共3页World Chinese Journal of Digestology

基　　金：湖北省教育厅资助项目;No.2000A43003~~

摘　　要：目的:用于治疗寄生虫病的酒石酸锑钾能抑制白血病细胞及淋巴瘤细胞生长,并诱导其凋亡.本文探讨其对人胃癌SGC-7901细胞株的作用及可能的机制. 方法:采用MTT法检测不同浓度酒石酸锑钾对胃癌SGC- 7901细胞的生长抑制作用;经Hoechst 33 258染色后用荧光显微镜观察细胞核的形态变化;DNA末端原位标记染色法、双染法(annexin-v/PI)和流式细胞术检测细胞凋亡. 结果:酒石酸锑钾能明显抑制胃癌细胞生长,抑制作用呈时间和剂量依赖性(方差分析,P<0.01);Hoechst 33 258 染色后荧光显微镜观察细胞核固缩,呈致密的强荧光;DNA 末端原位标记染色法、流式细胞仪检测均检测到凋亡细胞. 结论:酒石酸锑钾能诱导胃癌SGC-7901细胞凋亡.有望作为一种新的凋亡诱导剂用于胃癌的治疗.AIM: Potassium antimonyl tartrate (PAT), an antiparasitic agent, has rencently been shown to induce apoptosis of leukaemia cells. Its effects on human gastric cancer SGC-7901 cells and possible mechanism were investigated in this study. METHODS: The growth inhibition of cells induced by various concentrations of PAT in different time course was analyzed by using MTT assay. The nuclei were stained by Hoechst 33 258 and the morphologic changes were observed by fluorescence microscope. TUNEL staining, double-staining, and flow cytomery were used to detect apoptosis of cells. RESULTS: Growth inhibitory rates of SGC-7901 cells by PAT were significantly different in dose-and time-dependent manners (analysis of variance,P<0.01).Chromatin condensation and nuclear fragmentation were seen under fluorescence microscope in the cells treated with Hoechst 33 258, and apoptotic cells were also detected by flow cytometry and TUNEL staining. CONCLUSION: PAT can induce the apoptosis of SGC-7901 cells, and PAT may be a promising apoptosis-inducer in gastric cancer therapy.

关 键 词：酒石酸锑钾 胃癌 细胞凋亡 MTT法 流式细胞仪 肿瘤细胞 

分 类 号：R735.2[医药卫生—肿瘤]