塞莱西布体外对人类肝胃癌细胞生长的抑制作用  

Inhibitory effects of celecoxib on proliferation of human liver and gastric carcinoma cells in vitro

作　　者：樊菁[1] 窦科峰[1] 李开宗[1] 

机构地区：[1]中国人民解放军第四军医大学西京医院肝胆外科,陕西省西安市710032

出　　处：《世界华人消化杂志》2004年第3期523-526,共4页World Chinese Journal of Digestology

摘　　要：目的:研究塞莱西布在体外对人类肝癌7721细胞以及胃癌7901细胞的生长抑制作用. 方法:两种肿瘤细胞用含不同浓度(0,20,40,80,160 和320 μmol/L)的塞莱西布的培养液培养.应用MTT测定法来测定生长抑制率,电镜技术来观察细胞凋亡情况,免疫组化技术来检测细胞内Cox-2蛋白含量. 结果:塞莱西布对两种肿瘤细胞均具有生长抑制作用(塞莱西布320μmol/L时两种肿瘤细胞抑制率分别为49.1%和42.9%), 并呈现量-效关系.电镜下可观察到凋亡细胞.免疫组化发现细胞内环氧化酶的含量在处理前后有明显变化. 结论:在体外,塞莱西布抑制人类肝癌及胃癌细胞生长, 诱导他们产生凋亡,并且该作用与细胞内环氧化酶含量有密切关系.AIM: To study the inhibitory effects of celecoxib on proliferation of human hepatoma SMMC-7721 cells and gastric cancer SGC-7901 cells in vitro. METHODS: The two carcinoma cells were cultured with celecoxib at various concentrations (0, 20, 40, 80, 160 and 320 μmol/L). Growth suppression was detected with MTT colorimetric assay, cell apoptotic alterations were evaluated by transmission electron microscopy (TEM), and quantity of Cox-2 was evaluated by cytochemical staining. RESULTS: The inhibition of proliferation on two carcinoma cells was observed (49.1% and 42.9% by 320 nmol/L celecoxib). The inhibitory effect was dose-dependent. Apoptotic cells were observed under transmission electron microscope. The different quantities of Cox-2 protein in cells were observed by cytochemical staining. CONCLUSlON:Celecoxib inhibits proliferation, induces apoptosis of human two carcinoma cells in vitro, and the effects have close relation to the quantities of Cox-2 protein in cells.

关 键 词：塞莱西布 肝癌 胃癌 肿瘤细胞 肿瘤抑制 免疫组织化学 环氧化酶 炎症反应 细胞培养 

分 类 号：R735[医药卫生—肿瘤]