奥曲肽对人胃癌细胞株SGC-7901生长和凋亡的影响  

Effect of octreotide on growth and apoptosis of human gastric cancer cell line SGC-7901

作　　者：吕宾 徐毅 丁志山 

机构地区：[1]浙江中医学院附属医院消化科,浙江省杭州市310006

出　　处：《世界华人消化杂志》2004年第3期527-529,共3页World Chinese Journal of Digestology

摘　　要：目的:研究生长抑素类似物奥曲肽对人胃癌细胞株SGC- 7901生长和凋亡的作用. 方法:取人胃癌细胞株SGC-7901,体外培养后以处于对数生长的细胞制成单细胞悬液,加入不同浓度的奥曲肽,以MTT法进行细胞增生试验,并以不含药物的培养液为阴性对照;通过HE染色和电子显微镜观察细胞形态,通过流式细胞仪测定SGC-7901细胞周期和凋亡率. 结果:奥曲肽在一定浓度范围内(160-480 mg/L)对SGC-7901 细胞的生长具有抑制作用(与对照组比较,均P<0.001), 并存在量效关系和饱和性,最大抑制率为25.4%(奥曲肽浓度400 mg/L).奥曲肽可诱导SGC-7901细胞凋亡,HE 染色见奥曲肽作用48 h,细胞开始出现凋亡的形态学改变,72 h凋亡细胞明显增多,电镜下见有典型的凋亡小体出现.流式细胞仪分析可见Gl峰前出现亚二倍体峰,猾亡率为9.42±5.29%(P<0.05). 结论:奥曲肽在体外能抑制人胃癌细胞株SGC-7901细胞的生长,其作用与诱导肿瘤细胞凋亡有关.AIM: To observe the effects of somatostatin analog (octreotide) on the growth and apoptosis of gastric cancer cell line. METHODS: The proliferation of gastric cancer cell line SGC-7901 was determined in octreotide groups and control group by MTT. The cell morphology was observed under HE staining and electron microscopy. The cell cycle and apoptosis ratio were measured using flow cytometry analysis. RESULTS: The growth of gastric cancer cell line SGC-7901 was significantly inhibited by octreotide with 160-480 mg/L (vs controls, P <0.001), and the maximal inhibitory rate was 25.4%. Morphological variations of apoptosis were observed at 48 hours treated with octreotide by HE staining and apoptosis body was observed under electron microscope. Apoptotic rate was 9.42±5.29% (P<0.05). CONCLUSION: Octreotide can suppress the growth of gastric cancer cell line SGC-7901 and induce apoptosis of the cells.

关 键 词：奥曲肽 胃癌 SGC-7901细胞株 细胞凋亡 流式细胞仪 诱导凋亡 肿瘤抑制 

分 类 号：R735.2[医药卫生—肿瘤]