胰腺癌组织MGMT,hMLH_1和hMSH_2的表达意义  

Expression of MGMT, hMLH_1 and hMSH_2 and its clinopathological significance in pancreatic carcinoma tissues

作　　者：杨竹林[1] 邓星辉[1] 李永国[1] 钟德玝[1] 苗雄鹰[1] 

机构地区：[1]中南大学湘雅二医院肝胆疾病研究室,湖南长沙市410011

出　　处：《世界华人消化杂志》2004年第3期669-672,共4页World Chinese Journal of Digestology

摘　　要：目的:研究MGMT,hMLH1和hMSH2在慢性胰腺炎和胰腺癌组织中表达特征及其临床病理意义. 方法:胰腺癌(n=51)和慢性胰腺炎(m=10)手术切除标本经40 g/L中性甲醛固定后常规制作石蜡包埋切片,MGMT, hMLH1和hMSH2表达均采用常规ABC免疫组化法. 结果:胰腺癌MGMT,hMLH1和hMSH2表达阳性率(39.2%, 45.1%和50.9%)及其评分(1.8±1.4,1.7±1.6和1.9±1.7) 明显低于慢性胰腺炎阳性率(100.0%,100.0%和90.0%)及其评分(3.8±0.8,3.8±1.0和3.5±0.9),均有显著或高度显著性差异(P<0.05或P<0.01);转移病例三者表达阳性率及其评分较明显低于未转移病例,但均无显著性差异(P<0.05);三者表达与胰腺癌其他临床病理特征无明显关系. 结论:MGMT,hMLH1或hMSH2表达与胰腺癌发生及进展密切相关,均具有抑制胰腺癌发生及进展的作用.AIM: To study the expression of MGMT, hMLH1 and hMSH2 and their clinicopathological significances in the tissues of chronic pancreatitis and pancreatic adenocarcinoma. METHODS: The expression levels of MGMT, hMLH1 and hMSH2 were assayed by immunohistochemical method of avidin-biotin complex on the formalin-fixed and routinely paraffin-embedded sections of surgical resected specimen with chronic pancreatitis (n =10) and pancreatic carcinoma (n=51). RESULTS: The positive rates and the scores of MGMT, hMLH1 and hMSH2 were significantly higher in chromic pancreatitis than those of pancreatic carcinoma (MGMT: 100.0% vs 39.2%,3.8±0.8 vs1.8±1.4; hMLH1:100.0% vs45.1%, 3.8±1.0 vS 1.711.6; hMSH2: 90.0% vs50.9%, 3.5±0.9vs 1.9±1.7). The positive rates and the scores of MGMT, hMLHi and hMSH2 were significantly higher in well-differentiated adenocarcinomas than those of poorly differentiated ad-enocarcinomas (P<0.05 or P<0.01). The positive rates and the scores of MGMT, hMLHi and hMSH2 were higher in metastasis-free cases than those of ones with metastasis, but no statistic difference was found (P>0.05). There was also no difference among the expression of three proteins and the other clinicopathological characteristics of pancreatic carcinoma. CONCLUSION: The expression of MGMT, hMLHi or hMSH2 may be related to the carcinogenesis and progression, and have inhibitory effects on the carcinogenesis and progression of pancreatic carcinoma.

关 键 词：胰腺癌 癌组织 MGMT HMLH1 HMSH2 临床意义 ABC免疫组织化学法 肿瘤转移 预后 

分 类 号：R735.9[医药卫生—肿瘤]