体外培养的不同亚型肺癌细胞株差异蛋白的初步分析  

The preliminary analysis of distinct proteins for different subtypes of lung cancer cells lines

作　　者：夏梁[1] 蔡伟丽[1] 张丽娟[1] 孙一娜[1] 肖雪媛[1] 何大澄[2] 

机构地区：[1]北京师范大学生命科学学院细胞所高等学校蛋白质组学研究院,北京100875 [2]北京师范大学细胞生物学研究所,62208439

出　　处：《现代仪器》2004年第1期13-16,12,共5页Modern Instruments

基　　金：教育部科学技术重点(批准号:272006)资助项;国家自然科学基金(批准号:30370712)资助项目;国家"863"(批准号:2002AA232031)资助项目

摘　　要：分析体外培养的不同亚型肺癌细胞株蛋白质表达差异,筛选肺癌细胞的标志蛋白并与肺癌病人血清中的标志蛋白进行对比分析。采用SELDI(Surface Enhanced LaserDesorption/Ionization)蛋白质芯片技术检测了三种肺癌细胞株A549(肺癌)、Calu-6(腺癌)和PG(大细胞癌)以及人胚肺二倍体成纤维细胞(2BS)的蛋白质谱。结果显示与2BS细胞比较,肺癌细胞有24个蛋白质表达发生明显改变。Discovered the common biomarkers in lung cancer cell lines and detected the distinct proteins for different subtypes of lung cancer cell lines. Applied SELDI ( Surface Enhanced Laser Desorption/Ionization) proteinchip technique to detect three kinds of lung cancer cell lines; A549 and Calu - 6 (adenocarcinoma), and PG (large cell lung cancer) and human embryonic lung diploid fibroblast (2BS), Anion - exchange columns were used to fractionate the total protein of these cells with 4 designated pH washing solutions. Two different types of protein chip arrays, IMAC - Cu and WCX2, were used, protein chips were examined in PBSII - C Protein Chip Reader (Ciphergen Biosystem Inc) and the protein profiling was analyzed by Proteinchip Software 3.0.2. The results revealed twenty four peaks had significant difference between lung cancer cells and 2BS cells, ten of which were up - regulated in lung cancer cells, and the others were down- regulated. The results suggest that there are same distinct proteins in protein profiling between different subtypes of lung cancer cells and normal lung cessl, and the protein expression of different types of lung cancer cells is different,

关 键 词：蛋白质芯片 肺癌细胞株 差异蛋白 体外培养 SELDI 蛋白质谱 

分 类 号：R734.2[医药卫生—肿瘤] Q51[医药卫生—临床医学]