钩体LPS生物合成与装配途径及相关基因分析  

Pathway and related genes of lipopolysaccharide biosynthesis and assembly in Leptospira

作　　者：耿燕[1] 郭晓奎[2] 张怡轩[1] 缪有刚[1] 何建勇[3] 赵国屏[1] 

机构地区：[1]中国科学院上海生命科学院 [2]上海第二医科大学病原生物学教研室 [3]沈阳药科大学

出　　处：《中国人兽共患病杂志》2004年第1期5-9,4,共6页Chinese Journal of Zoonoses

基　　金：国家高技术研究发展计划 (863计划 ) (2 0 0 1AA2 2 3 0 3 1)

摘　　要：目的　预测问号钩体黄疸出血型赖株脂多糖 (LPS)合成、装配途径 ,并对其中几个关键基因的特征进行深入分析。方法　使用NCBI/BlastX ,ProDom/Blast ,Jpred2 ,TMHMM对问号钩体黄疸出血型赖株全基因组ORF基因所编码蛋白进行在线分析 ,并使用Bioedit,PepTool软件进行蛋白质一、二级结构分析。 结果　确定了脂质A和核心多糖生物合成基因lpxA、lpxB、lpxC、lpxD、lpxK、kdsA、kdsB、kdtA、lnt、htrB和O -抗原生物合成基因簇rfblocus等以及装配、跨膜转运中的基因rf bP、rfbX、rfc、rfaL、rfe绘制了LPS合成及装配途径示意图。并对合成和装配途径中几个关键的基因编码蛋白 (LpxA、Rfc、RfbX、RfaL)的二级结构、跨膜区域、结构域等进行了分析。结论　问号钩体黄疸出血型赖株LPS合成、装配途径与典型的革兰阴性菌 ,如大肠埃希菌K - 12相似 ,主要差异在于钩体特殊的脂肪酸代谢途径和O -抗原多糖结构。该研究为进一步探讨钩体LPS基因功能及其与致病性关系奠定基础。Objective To predict the pathway of synthesis and assembly of LPS for L.interrogans,and analyze the characterization of several involved important genes.Methods Using on-line database NCBI/Blast,ProDom/Blast,Jpred2,TMHMM et al to annotate genes on the chromosomes of L.interrogans,and using local software Bioedit and Pep Tool to analyze its characterization throughly.Results After Lipid-A precursor synthetical genes(lpxA,lpxB,lpxC,lpxD,lpxK,kdsA,kdsB,kdtA,lnt,htrB) were analyzed,as well as O-antigen synthetical rfb locus and its assembly genes (rfbP,rfbX,rfc,rfaL,rfe),the sketch map of LPS synthical and assembly pathway were finished,the secondary structure,transmembrane region,domain of the protein coded by genes:LpxA,Rfc,RfbX and RfaL were analyzed.Conclusion The pathway of synthesis and assembly for LPS in L.interrogans is similar to that of standard Gram-negative bacterium such as E.coli K-12.The difference was in metabolism of fatty acid and O-antigen structure.The annotation of LPS correlative genes using bioinformatics lay a foundation to further study on their function and relationship between LPS and pathogenesis by Leptospira.

关 键 词：钩体 LPS 生物合成 装配途径 基因分析 生物信息学 问号钩体 

分 类 号：R378[医药卫生—病原生物学]