恶性疟原虫Pf12基因重组质粒DNA接种诱导小鼠的免疫应答  

DNA Vaccine of Plasmodium falciparum:immune response of mice induced by injection of the recombinant plasmid DNA of Pf12

作　　者：马长玲[1] 余新炳[2] 单志新[3] 吴忠道[2] 徐劲[2] 卞国武[2] 胡旭初[2] 

机构地区：[1]广州医学院寄生虫学教研室,广州510182 [2]中山大学中山医学院寄生虫学教研室 [3]广东省人民医院心血管疾病研究所

出　　处：《中国人兽共患病杂志》2004年第1期31-34,共4页Chinese Journal of Zoonoses

基　　金：广东科技厅广东省团队项目基金资助

摘　　要：目的　观察重组质粒VR10 12 -Pf12DNA直接免疫接种诱导BALB/c小鼠所产生的免疫应答 ,分析Pf12基因的抗原性和作为疫苗抗原候选分子的可能性。方法　构建重组质粒VR10 12 -Pf12 ,直接免疫BALB/c小鼠 ,通过NK细胞杀伤活性、脾T淋巴细胞增殖水平、ELISA、体外抑虫试验测定 ,观察其诱导的细胞和体液应答以及体外抑虫效果。结果　重组质粒VR10 12 -Pf12免疫BALB/c小鼠 ,NK细胞杀伤活性、脾T淋巴细胞增殖水平明显高于对照组 (P <0 0 1) ,诱导小鼠产生的抗体水平明显增高 (P <0 0 1) ,免疫血清在体外能抑制恶性疟原虫的生长、发育。结论　重组质粒VR10 12 -Pf12DNA直接免疫接种诱导BALB/c小鼠产生一定水平的体液和细胞免疫应答 ,其免疫血清在体外对恶性疟原虫的生长、发育有明显的抑制作用。Objective To observe the level of immune responses of BALB/c mice induced by direct injection of the recombinant plasmid VR1012-Pf12 and to analyze its probability as DNA vaccine of Plasmodium falciparum.Methods The recombinant plasmid VR1012-Pf12 was constructed and immuned to the mice for 3 injections.NK cell killing activity,T lymphocyte proliferation activity,ELISA,inhibitory assay of the growth and development of P.f.in vitro were used to determine of Th-cell and humoral responses.Results At 60th days after 3 injections,NK cell killing activity and proliferation activity of splenic T lymphocyte in VR1012-Pf12 group were higher than that in VR1012 group(P<0.01) and NS group(P<0.01).The value of IgG in the mice increased and the titers of IgG were higer than that in VR1012 group(P<0.01) and NS group(P<0.01).The antiserum of VR1012-Pf12 group could inhibit the growth and development of erythrocytic stages of P.f.in vitro.Conclusion Direct injection with recombinant plasmid VR1012-Pf12 could elicite BALB/c mice to generate humoral and cell -mediated immune responses,the antiserum obtained could effectively inhibit the growth and development of P.f.in vitro.

关 键 词：恶性疟原虫 Pf12 基因重组质粒 DNA 接种 诱导 免疫应答 

分 类 号：R382.31[医药卫生—医学寄生虫学]