白细胞介素-2及其受体在癫痫患者发作中的变化和对癫痫大鼠的致惊作用  

The Changes of Interleukin-2 and Its Soluble Receptor in the Patients with Epilepsy and Effects on Convulsion in Genetically Epilepsy-prone Rats

作　　者：梁英武[1] 崔月华[1] 刘利平[2] 宋秀玲[2] 徐霖[2] 

机构地区：[1]济宁医学院儿科教研室,山东济宁272015 [2]济宁市第一人民医院儿内科,山东济宁272015

出　　处：《中国神经免疫学和神经病学杂志》2004年第1期26-28,共3页Chinese Journal of Neuroimmunology and Neurology

摘　　要：目的　探讨白细胞介素 -2 (interluekin-2 ,IL-2 )及其可溶性受体在癫痫患者发作中的变化和对癫痫大鼠的致惊作用。方法　采用 ELISA方法检测 5 8例癫痫患儿和 2 3例健康儿血清 IL-2及其可溶性受体的浓度 ,并进行分组比较 ;观察侧脑室注射 IL-2对遗传性癫痫易感大鼠 (P77PMC)惊厥发作的影响。结果　癫痫组血清IL-2及其可溶性受体浓度 [(2 5 .86± 5 .2 1 ) ng/ m L和 (75 8.2 6± 78.49) U/ m L ]明显高于对照组 [(1 3 .78± 3 .2 4)ng/ m L和 (3 2 5 .67± 3 4.5 8) U/ m L](P <0 .0 1 ) ,且发作期 [(3 0 .3 1± 6.77) ng/ m L和 (80 6.2 5± 1 1 2 .3 5 ) U/ m L]高于间歇期 [(2 0 .2 7± 4.66) ng/ m L和 (5 84.1 5± 5 7.85 ) U/ m L ](P <0 .0 1 ) ,脑电图异常者 [(2 7.1 8± 4.3 5 )ng/ m L和 (72 4.48± 78.5 6) U/ m L]高于正常者 [(2 0 .0 4± 3 .5 5 ) ng/ m L和 (62 1 .85± 77.45 ) U/ m L],但与发作类型、病程长短及经治疗与否无关 ;侧脑室注射 IL -2 (5 0 0 0 U/ L) 5～ 1 0μL后大鼠惊厥评分从 2 6升高到 3 6(P <0 .0 5 ) ,惊厥持续时间从 3 2 s延长到 65 s(P <0 .0 1 ) ,但对潜伏期无影响。结论　 IL-2在癫痫患者处于高水平状态 ,直接侧脑室注射 IL-2可加重大鼠惊厥程度 ,提示其参与癫?Objective To investigate the changes of interleukin-2 (IL-2) and its soluble receptor (sIL-2R),and effects on convulsion in genetically epilepsy-prone rat(P77PMC). Methods The serums IL-2 and sIL-2R of 58 epileptic patients[32 boys and 26 girls, aged 7 months-12 years, average (7.2±1.3) years] and 23 healthy children [control group, 14 boys and 9 girls, aged 5-12 years, average (8.2±1.5) years] were measured with ELISA and compared with groups divided, andtheeffectsofIL-2 on convulsionin P77PMCobservedwith injected by lateral ventricle. Results Itwasfoundthattheconcentrations of the serums IL-2 and sIL-2R of patients with epilepsy (25.86±5.21) ng/mL and (758.26±78.49) U/mL were significantly higher than the control group (13.78±3.24) ng/mL and (325.67 ±34.58) U/mL(P <0.01), otherwise attacking stage (30.31±6.77) ng/mL and (806.25±112.35) U/mL were significantly higher than resting stage (20.27±4.66)ng/mLand(584.15±57.85)U/mL(P <0.01),EEGnormalgroup(20.04±3.55) ng/mLand(621.85± 77.45) U/mLwaslowerthanEEGabnormal (27.18±4.35) ng/mL and (724.48±78.56) U/mL, but not being related to attacking type, the course or whether anticonvulsants had been taken, the convulsion scores of P77PMC increasedfrom26to36 (P <0.05) and prolonged duration from 32 seconds to 65 seconds (P <0.01) after injected IL-2 (5-10 μL). Conclusions These results indicate thatIL-2areina status of higher level in patients with epilepsy, and IL-2 injected directlybylateralventricle can aggravate convulsion intensity. It suggests that IL-2 is involved in epilepsy processes.

关 键 词：白细胞介素-2 受体 癫痫 ELISA 动物模型 惊厥 发病机制 小儿 

分 类 号：R742.1[医药卫生—神经病学与精神病学]