肺鳞癌中葡萄糖易化扩散转运蛋白-1过度表达与^(18)氟脱氧葡萄糖摄取的关系  

Preliminary research of the relationship between overexpression of facilitative glucose transporter-1 and fluorodeoxyglucose uptake in primary human lung squamous cell carcinoma

作　　者：汪涛[1] 孙玉鹗[1] 田嘉禾[2] 刘小兵[3] 姚树林[2] 王昆[1] 

机构地区：[1]解放军总医院胸外科,北京100853 [2]解放军总医院核医学科,北京100853 [3]解放军总医院病理科,北京100853

出　　处：《中华外科杂志》2003年第12期913-916,共4页Chinese Journal of Surgery

摘　　要：目的　了解葡萄糖易化扩散转运蛋白 1(Glut1)在肺鳞癌组织中过度表达与肿瘤对18氟脱氧葡萄糖 (FDG)摄取之间的关系。　方法　 1999年 4月～ 2 0 0 1年 3月对 2 3例肺鳞癌患者行全身正电子发射体层显像 (PET)检查 ,测定肿瘤对FDG的最大与平均标准摄取值 (SUVmax与SUVmean)及健侧正常肺组织的标准摄取值 (SUVlung)。所有肿瘤均手术切除。应用标准链霉菌抗生物素蛋白 过氧化物酶亲和 (SP)免疫组织化学方法 ,对肿瘤及正常支气管肺组织的Glut1表达情况进行检测 ,以染色阳性细胞率与染色强度 (染色强度 1～ 5 )表示。　结果　 2 3例肺癌组织均有Glut1过度表达 [阳性细胞率 (6 9± 18) % ,染色强度 4 6± 0 7],正常支气管肺组织无Glut1过度表达。肿瘤FDG摄取高于相应的正常肺组织 ,SUVmax、SUVmean与SUVlung分别为 8 33± 4 14、6 10± 3 0 0与 0 38± 0 13(P <0 0 1)。Glut1过度表达程度、FDG摄取、肿瘤大小之间呈正相关性 (P <0 0 1)。结论　肺鳞癌组织中的Glut1过度表达与其FDG摄取有直接的关系。Objective To assess the relationship between the overexpression of facilitative glucose transporter-1(Glut1) and fluorine-18 fluorodeoxyglucose(FDG) uptake in patients with primary lung squamous cell carcinoma. Methods From April 1999 to March 2001,23 patients with lung squamous cell carcinoma were imaged using FDG positron emission tomography(PET) before surgery.Their maximum and mean standard uptake values (SUVmax and SUVmean) of tumor and SUV of the normal lung(SUVlung) were measured.The expression of Glut1 of all the 23 cases was analysed in paraffin sections using SP immunohistochemistry. Results All the 23 tumors tested were Glut1 positive(69±18)% of tumor cell area was positive and staining intensity was 4.6±0.7.All tumors of the patients could be detected by FDG-PET.FDG uptake of tumor was higher than that of normal lung(P<0.01).SUVmax, SUVmean and SUVlung were 8.33±4.14,6.10±3.00 and 0.38±0.13 respectively.Correlations were found among Glut1 expression and FDG uptake and tumor size (P<0.01). Conclusions (1) Glut1 overexpression is universal in the lung squamous cell carcinoma. (2) SUV was higher in the lung squamous cell carcinoma than that of the normal lung tissue. (3) Glut1 expression and FDG uptake and tumor size appear to be correlated with each other in patients with lung squamous cell carcinoma.

关 键 词：肺鳞癌 葡萄糖易化扩散转运蛋白-1 过度表达 ^18氟脱氧葡萄糖 免疫组织化学 

分 类 号：R734.2[医药卫生—肿瘤]