体外孵育的非酶糖基化终产物诱导正常大鼠类糖尿病性视网膜血管病变研究  

Exogenous advanced glycosylation end products induce diabetes-like vascular dysfunction in normal rats: a factor for occurrence of diabetic retinopathy

作　　者：李治平[1] 许迅[1] 黄宇峰[1] 朱剑锋[1] 王晓珏[1] 胡宏慧[1] 何志平 

机构地区：[1]上海市第一人民医院眼科,200080 [2]香港眼科中心(嘉诺撒医院-明爱)

出　　处：《中华眼科杂志》2003年第6期352-356,共5页Chinese Journal of Ophthalmology

基　　金：国家自然科学基金资助项目 ( 3 9970 783 );上海市卫生系统优秀学科带头人培养计划资助项目 ( 97BR0 3 1)

摘　　要：目的　探讨外源性非酶糖基化终产物 (advancednon enzymaticglycationendproducts ,AGE)在血管壁的沉积与糖尿病性视网膜血管病变之间的关系。方法　将 16只健康两月龄SD大鼠 ,随机分为 4组 ,每组 4只鼠。正常对照组不做任何处理 ;糖尿病对照组应用AGE制作糖尿病模型 ;血清白蛋白 (ratserumalbumin ,RSA)组每日自鼠尾静脉注射RSA(4 0mg kg体重 ) ,连续注射 2周 ;AGE RSA组注射AGE RSA ,注射方法、剂量与RSA组相同。 2周后观察各组鼠视网膜毛细血管周细胞密度变化。结果　注射RSA组鼠视网膜毛细血管周细胞数平均为 (5 80± 0 4 8)个 每油镜视野 ,注射AGE组为(4 31± 0 34)个 每油镜视野 ,显示实验组毛细血管周细胞密度低于对照组 ,差异有显著意义 (F =7 16 4 ,P <0 0 1)。结论　外源性AGE注入正常大鼠会引起类糖尿病性视网膜血管病变 ,AGE可能是糖尿病性视网膜血管病变的独立致病因素之一。Objective To disclose the relationship between the deposition of advanced glycosylation end products (AGE) in the retinal vascular tissues and damage of retinal vessels in diabetic retinopathy. Methods Sixteen SD rats aged 2 months were divided into 4 groups, with 4 rats in each group. Rats in normal group received no treatment. Diabetes was induced by AGE in the diabetes group. Rat serum albumin (RSA, 40 mg/kg weight) was administered daily to healthy non-diabetic rats through tail veins for 2 weeks (RSA group). AGE-modified RSA was injected to rats in another group at the same route and dosage (AGE-RSA group). The number of pericytes in retinal capillary vessels was counted 2 weeks later. Results After two weeks continuous AGE treatment, the average amount of pericytes of capillary vessel per 10 microscope visual field (×100 magnification) in AGE group (4.31±0.34) was significantly less than that of RSA group (5.80±0.48) (P<0.01). Meanwhile, in the AGE-RSA group, AGE were identified in the retinal vascular tissues by immunohistochemical staining. Conclusion Injection of exogenous AGE into healthy rats induces vascular changes resembling those find in the diabetic retinopathy. AGE might be one of the independent pathogenic factors in the occurrence of diabetic retinopathy.

关 键 词：体外孵育 非酶糖基化终产物 大鼠 糖尿病 视网膜血管病变 AGE 血糖 

分 类 号：R774.1[医药卫生—眼科] R587.2[医药卫生—临床医学]