两种低强度微波辐射致兔晶状体上皮细胞改变的定量研究  

A quantitative study on early changes in rabbit lens capsule epithelium induced by low power density microwave radiation

作　　者：叶娟[1] 姚克[1] 鲁德强[2] 姜槐[2] 吴仁毅[1] 

机构地区：[1]浙江大学医学院附属第二医院眼科中心,杭州310009 [2]浙江大学医学院生物电磁学省重点实验室,杭州310009

出　　处：《中华眼科杂志》2003年第6期361-364,共4页Chinese Journal of Ophthalmology

基　　金：浙江省自然科学基金资助项目 ( 2 0 0 1SJSW0 0 3 )

摘　　要：目的　观察辐射强度为 5mW cm2 和 10mW cm2 的微波对兔晶状体上皮细胞的影响 ,探讨国际公认微波辐射安全域值的可靠性。方法　以辐射强度为 5mW cm2 (Ⅰ组 )和 10mW cm2 (Ⅱ组 )的微波分别连续照射兔眼 3h ,采用AnnexinⅤ 碘化丙啶荧光标记双染色流式细胞技术定量检测兔晶状体上皮细胞中正常细胞、早期凋亡细胞及继发性坏死细胞的数量 ,分别记录百分比。以对侧正常眼为对照。结果　Ⅰ组实验眼早期凋亡细胞的平均百分比为 (16 0± 6 8) % ,明显高于自身正常对照眼 ,差异有非常显著意义 (P <0 0 1) ;实验眼继发性坏死细胞的平均百分比与自身正常对照眼比较 ,差异无显著意义 (P >0 0 5 )。Ⅱ组实验眼早期凋亡细胞的平均百分比与自身正常对照眼比较 ,差异无显著意义 (P >0 0 5 ) ;实验眼继发性坏死细胞的平均百分比为 (16 2± 5 6 ) % ,明显高于自身正常对照眼 ,差异有显著意义 (P <0 0 5 )。 2个组实验眼早期凋亡细胞的平均百分比比较 ,差异无显著意义(P >0 0 5 ) ;继发性坏死细胞的平均百分比比较 ,Ⅱ组明显高于Ⅰ组 ,差异有显著意义 (P <0 0 5 )。结论　辐射强度为 5mW cm2 和 10mW cm2 的微波均可导致兔晶状体上皮细胞发生不可逆损伤 ,且微波辐射强度增加可加重损伤改变 ;5mW cm2 和 10mWObjective To test the safety of guidelines for limiting exposure to low power level microwave radiation and to try to explore the mechanisms of early damages induced by such radiation in rabbit lens capsule epithelial cells, using flow cytometry as a quantitative method of evaluation of these damages. Methods The rabbit eyes were exposed to 5 mW/cm 2 (group Ⅰ) and 10 mW/cm 2 (group Ⅱ) power densities of microwave for 3 h. Annexin Ⅴ-PI two-color flow cytometry was used to detect the changes of rabbits lens epithelial cells, apoptosis cells and secondary necrotic cells after radiation. The other eyes served as self-controls. Results In group Ⅰ, the percentage of early apoptosis cells in the experimental eyes was significantly greater than that of the controls (P<0.01), whereas no significantly difference in the percentage of secondary necrotic cells could be observed (P>0.05). In group II, there was no significant difference between the percentage of early apoptosis cells in experimental eyes and the controls (P>0.05), whereas the percentage of secondary necrotic cells in the experimental eyes was significantly greater than that of the controls (P<0.05).The percentage of secondary necrotic cells (16.2±5.6)% in group II is greater than that in group I (10.3±6.8)% (P<0.05), though there is no significant difference in the percentage of early apoptosis cells between these two groups (P>0.05). Conclusion Low power density of microwave radiation (5 mW/cm 2 and 10 mW/cm 2) can induce irreversible damage of rabbit lens epithelial cells in a dose-dependent manner. Therefore, the safety dosage criterion of microwave exposure needs further investigation.

关 键 词：低强度 微波辐射 兔 晶状体 上皮细胞 辐射强度 

分 类 号：R776[医药卫生—眼科]