胆囊结石患者血清、胆汁与胆囊黏膜中纳米细菌的分布研究  

Nanobacteria in serum,bile and gallbladder mucosa of cholecystolithiasis patients

作　　者：文宇[1] 李永国[1] 杨竹林[1] 王学军[1] 魏红[1] 刘威[1] 谭爱玲[2] 苗雄鹰[1] 王群伟[1] 黄生福[1] E.Olavi Kajander Neva Ciftcioglu 

机构地区：[1]中南大学湘雅第二医院肝胆疾病研究室,长沙410011 [2]中南大学基础医学院电镜中心 [3]Department of biochemistry and biotechnology

出　　处：《中华外科杂志》2003年第4期267-270,共4页Chinese Journal of Surgery

基　　金：教育部重大项目基金资助项目 (教技司 [2 0 0 1] 171号 )

摘　　要：目的　探讨纳米细菌在正常人群血清及胆囊结石患者血清、胆汁和胆囊黏膜中的分布情况。　方法　 ( 1)采用ELASA方法测定 338例正常人群和 76例胆囊结石患者血清中纳米细菌的感染情况 ;( 2 )对 5 7例胆囊结石和 18例非胆囊结石患者的胆囊胆汁进行纳米细菌培养 ,并采用免疫组化染色和透射电镜扫描进行鉴定 ;( 3)随机选取其中 40例胆囊结石患者胆囊黏膜进行免疫组化染色 ,观察相应胆汁和胆囊黏膜中纳米细菌分布的差异。　结果　 ( 1)正常人群和胆囊结石患者血清中纳米细菌感染率分别为 8 0 %和 31 6 % ,两者间差异有显著性 ( χ2 =2 9 90 ,P <0 0 5 ) ;( 2 ) 75例胆汁中有46例感染纳米细菌 ,阳性率为 6 1 3 % ,其中胆囊结石患者的胆汁纳米细菌的感染率为 6 1 4 % ,非胆囊结石患者的胆汁纳米细菌的感染率为 6 1 1% ,两者间并无统计学差异 ( χ2 =0 89,P >0 0 5 )。 ( 3) 14例胆囊结石患者胆囊黏膜感染纳米细菌 ,阳性率为 35 0 % ,其主要分布在黏膜或黏膜下层及钙化灶。　结论　正常人群血清中纳米细菌感染率为 8 0 % ,胆囊结石患者血清、胆汁和胆囊黏膜中存在着纳米细菌感染 ;胆囊壁的钙化或纤维化可能与纳米细菌感染有关。Objective To find the distribution of nanobacteria in the serum, bile and gallbladder mucosa of cholecystolithiasis patients. Methods The infection rate of nanobacteria was identified by ELASA in the serum samples from 338 healthy people and 76 patients with cholecystolithiasis (χ 2=0.89, P>0.05). Nanobacteria were cultured from the bile samples in 57 patients with cholecystolithiasis and 18 non-cholelithiasis patients and identified by immunohistochemical staining and TEM (χ 2=29.80,P<0.05). Forty samples of gallbladder mucosa randomly selected from the 57 cholecystolithiasis patients were identified by immunohistochemical staining and compared with the corresponding bile samples. Results The infection rate of nanobacteria was 8.0% and 31.6% for the serum samples of the healthy people and cholecystolithiasis patients, respectively. The positive rate of nanobacteria in the bile samples was 61.3% and there was no significant difference in the bile of the cholecystolithiasis patients and the control group(61.4% vs. 61.1%). Fourteen positive patients had infection of nanobacteria in the gallbladder mucosa , submucosa, and calcific field. Conclusions The infection rate of manobacteria was 8% in the serum samples from the healthy people. There are nanobacteria in the serum, bile, and gallbladder mucosa. The infection of the nanobacteria may result in calcification and fibrosis of the gallbladder.

关 键 词：胆囊结石 血清 胆汁 胆囊黏膜中纳米细菌 分布 研究 免疫组织化学 

分 类 号：R575.62[医药卫生—消化系统]