变性纤维银浸染法在神经组织培养中的应用  

The Application of silver impregnation method of degenerated fibers in nerve tissues culture

作　　者：王丽琴[1] 吴淑玉[1] 刘瑞春[1] 宋学琴[1] 李春岩[1] 

机构地区：[1]河北医科大学第二医院神经内科,050000

出　　处：《脑与神经疾病杂志》2003年第6期333-334,362,共3页Journal of Brain and Nervous Diseases

摘　　要：目的:以银浸染法显示神经组织培养中的变性纤维。方法:取大鼠胚胎脊髓前角细胞(SAMNS)培养,5d后加入急性运动性轴索型神经病(AMAN)病人血清,干预12h后用铜-银浸染法染色。结果:变性纤维呈黑色或棕黑色,正常纤维呈淡黄色。结论:银浸染法能识别神经组织培养中变性的纤维。Objective: To show degenerated fibers in nerve tissues culture by silver impregnation method. Methods: Spinal anterior motor neurons (SAMNs) were cultured for 5 days from rat embryo. The cultured neurons were exposed in the AMAN serum for 12 hours. Then the cultured neurons were stained by copper-silver impregnation method. Results: The degenerated fibers were stained black or brown black, while the normal fibers were stained light yellow. Conclusions: Silver impregnation could distinguish the degenerated fibers in nerve tissue culture.

关 键 词：变性纤维 银浸染法 神经组织 培养 急性运动性轴索型神经病 

分 类 号：R741[医药卫生—神经病学与精神病学]