全膀胱辐照光动力学阻断膀胱癌变的实验研究  

A Study on Inhibition of Precancerous Lesions by Whole-Bladder-Wall PDT Laser Irradiation in Rats

作　　者：范贤骏 谢建国[1] 张金生[1] 李泰生[1] 刘玉英[1] 宋序彦 张力军 林培中[1] 

机构地区：[1]中国医学科学院肿瘤研究所,100021

出　　处：《中国激光医学杂志》1992年第2期93-95,共3页Chinese Journal of Laser Medicine & Surgery

摘　　要：膀胱癌常表现为多灶性,如何治疗癌前病变是减少复发、提高治愈率的关键。我们根据先前的研究结果,应用激光全膀胱辐照加血卟啉衍生物的方法,对膀胱癌前病变进行阻断性治疗研究。将大鼠随机分为2组,组1作为对照仅给予致癌物亚硝胺(BBN);组2除给予BBN外,还在2组停止BBN后进行全膀胱辐照光动力学处理一次。致癌物BBN的剂量为30mg/kg体重,每周3次共24周。其后,组2鼠腹腔注射血卟啉衍生物HpD,20mg/kg体重,48小时后用激光辐照全膀胱壁,光能量密度约为40J/cm^2。第11个月后处死动物进行病理组织学检查。结果表明,膀胱癌的发病率治疗组比对照组降低69.7％(x^2=6.09,P<0.05)。作者认为,如果进行2次以上全膀胱激光辐照光动力学处理,膀胱癌变率可能会进一步降低。上述结果为临床预防膀胱癌的复发。Bladder cancer often appears with multifoci, and the visible tumor foci usually coexist with invisible precancerous lesions which are difficult to treat by conventional therapies. Based on our previous studies we designed this study on inhibitory therapy of precancerous lesions in rat's bladder by whole-bladder-wall laser-irradiation plus hematoporphyrin derivative (HpD). The rats were divided at random into two groups. Group 1 was control, the rats received N-butyl-N-(4-hydroxybutyl) nitrosamine (BBN) only. Group 2 was used for therapy, in which the rats received BBN and Photodynamic therapy(PDT), The rats in both groups were given BBN three times a week for 24 weeks in a dose of 30mg/kg body weight. After 24 weeks the rats in group 2 were treated with the whole-bladder-wall laser-irradiation plus HpD, the dosage of HpD was 20mg/kg b.w. intraperitoneally 48h before, the estimated energy density of the irradiated area was 40 J/cm^2. The period of experiment was 11～13 months, at that time the rats wore killed for histopathological examination. The results showed that the incidence of bladder cancer in group 2 was reduced by 69.7% (X^2=6,09, P<0.05) as compared with control group. We inferred that the inhibition rate of bladder cancer will be increased if the procedure of whole-bladder-wall laser-irradiation plus HpD is carried out two or more times. This re. sult provides a useful reference for clinical trial in preventing precancerous lesions from developing an entity.

关 键 词：染料激光器 光动力学疗法 膀胱肿瘤 

分 类 号：R737.140.5[医药卫生—肿瘤]