多巴胺D_2受体TaqI A多态性与海洛因渴求程度的关联分析  

Relationship between heroin craving level and DRD_2 recepto gene-TaqI A polymorphism

作　　者：邵春红[1] 江开达[2] 李一峰[3] 赵敏[2] 宋立升[2] 徐一峰[2] 金力[3] 周伟航 刘培勇 秦海鸥 

机构地区：[1]复旦大学附属华山医院精神医学教研室,上海市精神卫生中心,200040 [2]上海市精神卫生中心 [3]复旦大学生命科学院遗传所 [4]上海市禁毒办 [5]上海市海运强制戒毒所

出　　处：《上海精神医学》2004年第1期10-14,共5页Shanghai Archives of Psychiatry

摘　　要：目的　探讨多巴胺D2 受体TaqIA多态性与线索诱发海洛因渴求程度的关系。 方法　380名海洛因依赖者接受环境诱发渴求实验 ,用PCR RFLP技术检测DRD2 受体TaqIA多态性 ,比较不同基因型与线索暴露前后渴求程度的关系。结果　强制戒毒被试者基因型与暴露前后渴求程度未见显示差异 (P >0 0 5 )。自愿戒断被试者基因型与诱发后渴求分值、暴露前后渴求分差值的差异有显著性 (F =4 5 2 3,P =0 0 12 ;F =3 936 ,P =0 0 2 1) ,A1/A1基因型被试者暴露后渴求程度高于A2 /A2 (P =0 0 2 7,P =0 0 19)和A1/A2 (P =0 0 32 ,P =0 0 35 )被试者。三者基因型在成瘾、加量和海洛因使用时间差异上有显著性 (P =0 0 14 ,P =0 0 0 1,P =0 0 0 4 )。A1/A1基因型个体成瘾时间和加量时间小于A2 /A2 (P =0 0 0 7,P =0 0 0 1)和A1/A2 (P =0 0 2 3,P =0 0 0 1)基因型个体 ,但吸毒时间大于A1/A2 (P =0 0 0 3)和A2 /A2 (P =0 0 0 2 )基因型个体。结论　DRD2 受体基因TaqIA多态性可能与环境诱发海洛因渴求程度易感性和成瘾、加量及毒品使用时间有关 ,A2 + (A2 /A2 ,A1/A2 )基因型可能在海洛因依赖中起保护作用 ,而A2 -(A1/A1)基因型则可能具有促进作用。Objective: To investigate the relationship between DRD 2 receptor TaqI A polymorphism and cue-induced craving level of heroin. Methods: Genotype of DRD 2 receptor TaqI A using PCR-RFLP were detected in 380 subiecrs with dependence. The relationship between craving level before and after and the genotype were compared. Results: No significant correlation has been found between craving level before and after cue-exposure and genotype in cue exposure forced detoxification subjects (P>0.05). However, significant correlation has been detected between genotype and cue-induced craving level and difference of crowing level befare and after cue exposure in volunteer detoxification subjects (F=4.523, P=0.012; F= 3.936, P=0.021). Craving level after cue-exposure in A 1/A 1 genotype is higher than that in A 1/A 2(P= 0.032, P=0.035) and A 2/A 2(P=0.027, P=0.019) genotype. Significant difference exists inthree different in terms of addictive time, increasing-dosage time and heroin-using time (P=0.014, P=0.001, P= 0.004). Addictive time and time of increasing-dosage in A 1/A 1 subjects are shorter than that in A 2/A 2(P= 0.007, P=0.001) and A 1/A 2(P= 0.023, P=0.001) subjects. But heroin-using time in A 1/A 1 individuals is longer than that in A 2/A 2 and A 1/A 2 individuals (P=0.002, P=0.003 respectively). Conclusion: DRD 2 receptor gene TaqI A polymorphism may have association with susceptibility of cue-induced craving level and some addictive related indexes such as addictive time, increasing-dosage time and heroin-using time. A 2 +(A 1/A 2, A 2/A 2) genotype may play a protective role in heroin dependence, whereas A 2 -(A 1/A 1) genotype take a precipitating effect.

关 键 词：多巴胺D2受体 TaqIA 基因多态性 海洛因渴求 海洛因依赖 戒毒 

分 类 号：R749.6[医药卫生—神经病学与精神病学]