δ、μ和κ型阿片肽受体在人类血管壁组织中的表达  

EXPRESSION OF OPIOID(δ,μ,κ )RECEPTORS IN THE WALL OF HUMAN BLOOD VESSELS

作　　者：李志 聂文洁 李柏健[3] 赵洁[3] 纪祥瑞[3] 

机构地区：[1]青岛万杰医院心脏外科,山东青岛266101 [2]青岛万杰医院病理科 [3]青岛大学医学院附属医院病理科

出　　处：《青岛大学医学院学报》2004年第1期34-36,共3页Acta Academiae Medicinae Qingdao Universitatis

摘　　要：①目的　检测人类周围血管组织中三种阿片肽受体存在与分布情况 ,探讨其意义。②方法　应用免疫组化技术检测了 6 0例含血管的新鲜组织 (子宫 2 0例 ,肺 1 2例 ,卵巢 1 2例 ,脾 1 0例 ,甲状腺 6例 )冷冻切片中δ型阿片肽受体 (DOR)、μ型阿片肽受体 (MOR)和κ型阿片肽受体 (KOR)的表达情况。以血管壁着棕黄色者判为阳性。③结果　DOR、MOR、KOR的表达阳性率分别为 5 3.3%、5 6 .7%和 6 8.3% ,三者比较差异无显著性 (χ2 =3.0 88,P >0 .0 5 ) ,且DOR、MOR和KOR在不同器官中的表达均无差异 (χ2 =6 .6 1 7～ 7.81 5 ,P >0 .0 5 )。在子宫、卵巢、脾、甲状腺血管内膜及肺泡壁毛细血管均有棕黄色着色 ;在子宫、卵巢和甲状腺可见部分组织细胞亦着色。④结论　KOR、DOR、MOR在人类周围血管组织确有表达 ,并多在血管内膜着色 ;三种亚型受体间阳性表达率无明显差异 ;Objective To detect the existence and distribution of opioid receptors in human peripheral blood vessels. Methods An immunohistochemical (IHC) technique was used to detect KOR, MOR and DOR expressions in 60 human tissues containing blood vessels with 20 from uterus, 12 from lung, 12 from ovary, 10 from spleen, and 6 from thyroid. The appearance of brown color in the blood vessel wall was regarded as positive. Results The positive rates of KOR, MOR and DOR were 68.3% , 56.7% and 53.3%, respectively, without any significant difference between them (χ 2=3.088, P>0.05). The positive rates of the receptors in different tissues showed no significant difference (χ 2=6.617-7.815, P>0.05).The brown color was stained in all vessel walls of the uterus, ovary, spleen, thyroid and lung; part of the tissue cells of the uterus, ovary and thyroid was also stained. Conclusion Opioid receptors do express in the endothelium of human peripheral blood vessels. No significant difference exists in the positive expression of the three receptors. The expression of the same receptor in different tissues shows no evident difference.

关 键 词：阿片样受体 血管 免疫组织化学 周围血管组织 

分 类 号：R361.2[医药卫生—病理学]