CO_2激光辐射总状毛霉发酵产蛋白酶优化条件  

Study on optimal culturing of high proteinase-producing mucor racemosus mutated by CO_2 laser

作　　者：李佳[1] 刘鑫[1] 刘克武[1] 国锦林[1] 张勇侠[1] 肖舸[1] 黄新河[1] 

机构地区：[1]四川大学生命科学学院,成都610064

出　　处：《激光技术》2004年第2期128-130,共3页Laser Technology

摘　　要：为了选育高产食品蛋白酶菌株并研究其最佳发酵条件 ,从豆腐乳中分离出总状毛霉MR137,用CO2 激光辐射诱变。激光功率 10W ,扩束光斑直径 0 .4cm ,照射距离 2 2cm ,照射时间 4 0s。经初筛、复筛 ,选育出高产蛋白酶变异株 (MR137 3)。经传代培养 ,MR137 3具有稳定的高产蛋白酶活性。对MR137 3的固体发酵条件进行研究发现 ,培养温度 30℃ ,培养时间 72h ,培养基pH6 .0 ,培养基含水量 6 5 % ,是MR137Mucor racemosus 137 was isolated from fermented bean curd and brought to mutation by radiation of CO 2 laser. The duration for radiation was 40s with distance of 22cm, and the diameter of laser beam was 0.4cm. High proteinase-producing mutant MR137-3 was obtained after screening cultures. The MR137-3 was found to have stable proteinase-producing activity in descendant cultures. The optimal culturing could be obtained at 30℃, pH6.0, culturing duration of 72h, with water content of 65% of culture medium.

关 键 词：CO2激光 总状毛霉 发酵 蛋白酶 优化条件 

分 类 号：O658.13[理学—分析化学]