猪流行性腹泻病毒嵌套式RT-PCR检测方法的建立  

Establishment of Nested RT-PCR Diagnostic Method for Porcine Epidemic Diarrhea Virus

作　　者：张素芳[1] 贾赟[2] 王敏秀[1] 倪艳秀[3] 何孔旺[3] 陈溥言[1] 

机构地区：[1]南京农业大学农业部动物疫病诊断与免疫重点开放实验室,江苏南京210095 [2]沈阳农业大学动物医学院,辽宁沈阳110161 [3]江苏省农业科学院农业部畜禽疫病诊断重点开放实验室,江苏南京210014

出　　处：《中国病毒学》2004年第2期174-176,共3页Virologica Sinica

摘　　要：Two pairs of primers were designed according to the N gene sequence of PEDV-CV777 strain in Genebank (No. AF353511). PCR amplification product of outer-primers was 1328bp, and the inter-primers amplification product was 538bp. With the primers, a nested PCR assay was established to detect PEDV. This method was sensitive and specific and could be used in PEDV diagnosis and epidemiological investigation.Two pairs of primers were designed according to the N gene sequence of PEDV-CV777 strain in Genebank (No. AF353511). PCR amplification product of outer-primers was 1328bp, and the inter-primers amplification product was 538bp. With the primers, a nested PCR assay was established to detect PEDV. This method was sensitive and specific and could be used in PEDV diagnosis and epidemiological investigation.

关 键 词：猪流行性腹泻病毒 嵌套式RT-PCR检测方法 PED 诊断方法 分子生物学技术 

分 类 号：S854.4[农业科学—临床兽医学]