血清白蛋白的铜Ⅱ-乙酰丙酮络合吸附波法测定  

Determination of Proteins Based on Polarographic Adsorptive Wave of Copper-Acetylacetone Complex

作　　者：郑石英[1] 罗登柏[1] 

机构地区：[1]中南民族大学化学与生命科学学院,武汉430074

出　　处：《分析化学》2004年第4期456-458,共3页Chinese Journal of Analytical Chemistry

基　　金：湖北省自然科学基金 (No .99J0 63 );中南民族大学自然科学基金 (No .YZZ0 3 0 0 3 )资助项目

摘　　要：在pH 6 .8的磷酸盐缓冲溶液中 ,血清白蛋白与铜 乙酰丙酮络合物反应作用生成一种在汞电极上吸附性很弱的铜 血清白蛋白惰性络合物 ,使铜 乙酰丙酮络合物在 - 0 .2 8V(vs .SCE)络合吸附波还原峰电流降低 ,电流降低值与 1～ 30mg/L范围内牛血清白蛋白 (BSA)或 1～ 32mg/L范围内人血清白蛋白 (HSA)呈线性关系 ;检出限分别为 0 .5和 0 .6mg/L。运用该法测定了人血清样品中蛋白质含量 。The interaction of protein with Cu(II)-acetylacetone complex in pH 6.8 NaH2PO4-Na2HPO4 buffer solution has been studied by single sweep polarography. Adding bovine serum albumin (BSA)/human serum albumin (HSA) into the Cu(II)-acetylacetone complex solution results in the formation of an electrochemical non-active complex of Cu(II)- BSA/HSA with much less adsorption than Cu(II)-acetylacetone complex at the mercury electrode, and causes the current decrease of the adsorptive peak of Cu(II)-acetylacetone complex. The Deltai(p) is proportional to the protein concentration from 1 mg/L to 30 mg/L for BSA and from 1 mg/L to 32 mg/L for HSA. The detection limit is 0.5 mg/L for BSA and 0.6 mg/L for HSA. The method was used for the determination of total protein in human serum sample. The result is satisfactory.

关 键 词：血清白蛋白 铜-乙酰丙酮络合物 吸附波法 铜-血清白蛋白络合物 单扫描极谱法 含量测定 络合吸附波 

分 类 号：O657.61[理学—分析化学]