mRNA致敏树突细胞对胃癌的免疫治疗作用研究  

Immunotherapy of dendritic cells transfected with mRNA of gastric cancer cells in carried-tumor mice

作　　者：罗治彬[1] 徐采朴[2] 朱高友 张朋斌[4] 郭朝华[5] 罗元辉[2] 房殿春[2] 罗成基[5] 

机构地区：[1]中国人民解放军第三军医大学大坪医院肿瘤中心,重庆市400042 [2]中国人民解放军第三军医大学西南医院全军消化内科中心,重庆市400038 [3]重庆市垫江高安中心医院,重庆市408306 [4]中国人民解放军第三军医大学新桥医院消化内科,重庆市400037 [5]中国人民解放军第三军医大学全军复合伤研究所,重庆市400038

出　　处：《世界华人消化杂志》2004年第1期13-15,共3页World Chinese Journal of Digestology

摘　　要：目的:了解DC在胃癌胃黏膜中的功能状态,探讨DC用于胃癌等实体瘤防治的可能性,旨在为胃癌的治疗探索一条新的途径. 方法:利用致敏DC治疗胃癌,以观察其对荷胃癌小鼠的免疫治疗效果. 结果:荷胃癌小鼠经胃癌细胞RNA致敏的DC治疗后,小鼠腹水产生率、肿瘤转移率、动物的存活率、平均瘤体质量等指标分别为75%(6/8)、25%(2/8)、75%(6/8)、(2.04±0.33 g),均优于对照组(P<0.01);局部组织中IL-12、IL-18 和IFN γ的基因表达水平都明显升高(P<0.01);肿瘤局部CD4+、CD8+细胞数量分别为0.71±0.25/μm2和0.67±0.22/μm2, P<0.05),TIL细胞毒活性明显增强. 结论:胃癌RNA致敏的DC,能促进胃癌抗原的提呈并启动和增强局部的抗瘤免疫功能,改善临床症状和生存质量, 对荷胃癌小鼠具有一定的免疫治疗效果.AIM: To probe into the possibility of dendritic cells (DC) in preventing solid carcinomas such as gastric cancer (GC). METHODS: Immunotherapy was proformed by DC sensitized by mRNA of gastric cancer cells. RESULTS: The indices such as the production rate of as-cites of mice (75%), the metastasis rate of tumor (25%), the survival rate of animals (75%) and the average weight of tumor (2.04±0.33 g) showed that the condition of the mice treated by DC was better than that in the control group (P<0.01). And the level of gene expression of IL-12, IFNγ and IL-18 in local tumor tissue was significantly raised (P<0.01) and the amount of local CD4+(0.71±0.25/μm2) and CD8+ (0.67±0.22/μm2) cells was increased (P<0.05) and the cytotoxicity of TIL was remarkably enhanced. CONCLUSION: DC transfected with mRNA isolated from mouse tumor cells may effectively control the growth of tumor and ameliorate the symptoms, suggesting that a certain therapeutic efficiency for mice carrying tumor can be realized.

关 键 词：胃癌 免疫治疗 mRNA致敏树突细胞 防治 

分 类 号：R735.2[医药卫生—肿瘤]