Two useful methods for evaluating antihypertensive drugs in conscious freely moving rats  

作　　者：Ding-fengSU Li-pingXU Chao-yuMIAO He-huiXIE Fu-mingSHEN Yuan-yingJIANG 

机构地区：[1]DepartmentofPharmacology，SecondMilitary,MedicalUniversity,Shanghai200433,China

出　　处：《Acta Pharmacologica Sinica》2004年第2期148-151,共4页中国药理学报（英文版）

基　　金：Project supported by the High Tech Research;Develop-ment (863) program (2002 AA2Z346C); the National Natu-ral Science Foundation of China (39670831 and 30070871).

摘　　要：AIM: Computerized analysis of blood pressure in conscious freely moving rats is a sound technique for physiologi- cal and pharmacological studies. The present work, based on this technique, was designed to introduce two useful methods for the evaluation of antihypertensive drugs in conscious spontaneously hypertensive rat (SHR). They were the directly intragastric administration of drugs and modified probability sum test for evaluating the synergism of the combination of two drugs. METHODS AND RESULTS: (1) Directly intragastric administration was used in conscious rats. A catheter was inserted into stomach immediately after arterial catheter insertion. Three days after operation, blood pressure was recorded and drug might be given intragastically via the gastric catheter. (2) Modi- fied probability sum test was used to evaluate the synergism of two drugs. The formula was: q=PA+B/(PA+PB?PA×PB). With this method, it was obtained: q=1.32 for the effects of the combination of atenolol and nitrendipine (20 mg/kg+10 mg/kg) on systolic blood pressure; q=1.41 for the effects of the combination of atenolol and amlodipine (10 mg/ kg+1 mg/kg) on systolic blood pressure. CONCLUSION: The two methods introduced by the present work will be important and useful for antihypertensive drug evaluation in conscious freely moving rats.AIM: Computerized analysis of blood pressure in conscious freely moving rats is a sound technique for physiologi- cal and pharmacological studies. The present work, based on this technique, was designed to introduce two useful methods for the evaluation of antihypertensive drugs in conscious spontaneously hypertensive rat (SHR). They were the directly intragastric administration of drugs and modified probability sum test for evaluating the synergism of the combination of two drugs. METHODS AND RESULTS: (1) Directly intragastric administration was used in conscious rats. A catheter was inserted into stomach immediately after arterial catheter insertion. Three days after operation, blood pressure was recorded and drug might be given intragastically via the gastric catheter. (2) Modi- fied probability sum test was used to evaluate the synergism of two drugs. The formula was: q=PA+B/(PA+PB?PA×PB). With this method, it was obtained: q=1.32 for the effects of the combination of atenolol and nitrendipine (20 mg/kg+10 mg/kg) on systolic blood pressure; q=1.41 for the effects of the combination of atenolol and amlodipine (10 mg/ kg+1 mg/kg) on systolic blood pressure. CONCLUSION: The two methods introduced by the present work will be important and useful for antihypertensive drug evaluation in conscious freely moving rats.

关 键 词：AMLODIPINE ATENOLOL blood pressure NIFEDIPINE NITRENDIPINE spontaneously hypertensive rat 

分 类 号：R972.4[医药卫生—药品] R-332[医药卫生—药学]