同源盒基因Msx-1、Msx-2和Dlx-2在小鼠下颌第一磨牙发育阶段的表达  

Expression of homeobox gene Msx-1,Msx-2 and Dlx-2 during murine mandibular first molar development

作　　者：马丽[1] 陈智[2] 宋光泰[1] 樊明文[2] 张旗[2] 王志峰[2] 

机构地区：[1]武汉大学口腔医学院儿童牙科,430079 [2]武汉大学口腔医学院口腔生物医学工程教育部重点实验室,430079

出　　处：《中华口腔医学杂志》2003年第6期429-431,C004,共4页Chinese Journal of Stomatology

基　　金：国家自然科学基金资助项目 (3 0 1710 11)

摘　　要：目的　观察同源盒基因Msx 1、Msx 2和Dlx 2mRNA在小鼠下颌第一磨牙发育阶段的表达。方法　取胎龄E11～E18和新生P1～P3小鼠的头或下颌 ,制备 5 μm连续冠状切片。体外转录合成地高辛标记的Msx 1、Msx 2和Dlx 2RNA探针。原位杂交后观察Msx 1、Msx 2和Dlx 2在小鼠下颌第一磨牙中的表达。结果　在牙胚发育过程中 ,Msx 1转录信号始终只在间质细胞中观察到 ,在上皮细胞中呈阴性。Msx 2和Dlx 2在牙源性上皮和间质中均表达 ,在磨牙发育起始期二者表达相似 ,但在随后的牙胚发育过程中 ,它们出现不同的表达特征。结论　牙胚发育过程中 ,Msx 1、Msx 2和Dlx 2有不同的表达特征 ,可能共同参与调控上皮和间质间的相互作用。Objective To observe the expression of homeobox gene Msx-1, Msx-2 and Dlx-2 during murine mandibular first molar development. Methods The murine heads or mandibles on embryonic days 11-18(E11-18)and postnatal day 1-3 (P1-3) were removed , fixed and embedded, 5 μm serial sections were cut in the coronal plane. Msx-1, Msx-2 and Dlx-2 RNA probes were synthesized by in vitro transcription and labeled with digoxigenin. Msx-1, Msx-2 and Dlx-2 mRNA expression was observed after in situ hybridization. Results During molar development Msx-1 transcripts appeared only in mesenchymal cells, not in epithelial cells. Msx-2 and Dlx-2 both expressed in the epithelial and mesenchymal cells. At the initiation stage of the molar development Msx-2 and Dlx-2 had similar expression. At the bud stage (E13-14) Msx-2 mRNA signaling was intensive in the enamel organ and slight in the dental mesenchyme; Dlx-2 signaling was stronger in the dental papilla. At cap stage (E15-16) Msx-2 showed prominent mRNA signaling in enamel knot and Dlx-2 was maximal in the dental papilla. At the late bell stage (P2-3) Msx-2 transcripts were observed in odontoblasts but not labeled in ameloblasts, and Dlx-2 transcripts appeared in ameloblasts but no labeling was seen in odontoblasts. Conclusions Msx-1, Msx-2 and Dlx-2 are expressed in various patterns during murine mandibular first molar development, suggesting they possibly play a role in the interaction between the epithelium and mesenchyme during the molar development.

关 键 词：同源盒基因 Msx-1 Msx-2 Dlx-2 小鼠 下颌第 一磨牙 牙发育阶段 基因表达 牙胚发育 

分 类 号：R780.2[医药卫生—口腔医学]