用基因芯片研究同一血管瘤增生和消退期差异表达基因  

Identification of differentially expressed genes in proliferating and involuting hemangiomas by cDAN microarray analysis of gene-expression profiles

作　　者：张莉[1] 王炜[1] 祁佐良[1] 董佳生[1] 宋怀东[2] 杨劲松[3] 林晓曦[1] 

机构地区：[1]上海第二医科大学附属九院整形外科,200011 [2]上海第二医科大学附属瑞金医院分子中心 [3]上海第二医科大学生化教研室

出　　处：《中华整形外科杂志》2003年第6期452-455,共4页Chinese Journal of Plastic Surgery

摘　　要：目的　利用基因表达谱芯片技术寻找血管瘤增生期到消退期之间差异表达的基因 ,初步探索血管瘤增生与自然消退的分子机制。方法　将 4 0 96条cDNA用点样仪点在特制玻片上制备成表达谱芯片 ;将同一血管瘤患儿的增生期和消退期瘤体组织的mRNA逆转录为cDNA、分别标记Cy3和Cy5两种荧光 ,制备成cDNA探针 ,与表达谱芯片杂交 ,通过计算机扫描、数据处理筛选出差异表达的基因。结果　差异表达的基因有 194条 ,其中 115基因条上调 ,79条下调 :①增生期一些细胞因子和生长因子高表达 ;②消退期细胞凋亡因子表达增加 ;③血管形成和原癌基因参与血管瘤的增生 ;④线粒体激活的细胞凋亡通路和Wnt β catenin通路可能与血管瘤的发生和发展有关。 结论　血管瘤的发生可能是细胞增殖和凋亡比例失调所致。Objective To investigate the differentially expressed genes of proliferating and involuting hemangiomas by cDNA microarray analysis of gene-expression profiles in an effort to identify the key disease-related genes. Methods Samples were processed from total RNA and purified to mRNA, which was reverse-transcripted and hybridized onto Biodoor Genechip expression microarrays. Analyses were performed to determine the consensus pattern of gene expression in the proliferating and involuting stages of the same hemangioma and the changes in the expression level. Results In proliferating hemangioma, 79 genes were overexpressed, and 115 genes were underexpressed in comparison with the involuting hemangioma. Some cytokines and growth factors such as neurotensin, Nov, CYR6, keratinocyte growth factor, interleukin-10 were overexpressed in proliferative hemangioma. In involuting hemangioma, apoptotic factors such as bcl-2 binding component, cytochrome C were overexpressed. The overexpression of Nov, CYR6, c-myc implied that angiogenesis and oncogenes might participate in the pathogenesis of hemangiomas. Mitochondria activated apoptotic passage (cytokines, bcl-2, cytochrome C) and Wnt/beta-catenin passage(Frizzled, beta-catenin, c-myc)were involved. Conclusion The development of hemangiomas may be the results of imbalance of cell proliferation and apoptosis. ;

关 键 词：基因芯片 表达谱 血管瘤 细胞增殖 细胞凋亡 差异表达基因 

分 类 号：R732.2[医药卫生—肿瘤]