竹叶黄酮作为抗衰老护肤因子的应用基础研究  

PRIMARY STUDIES ON BAMBOO-LEAF-FLAVONOIDS USED AS ANTI-AGING FACTOR FOR SKIN PROTECTION

作　　者：张英[1] 沈建福[1] 俞卓裕[1] 陆柏益[1] 楼鼎鼎[1] 

机构地区：[1]浙江大学生物系统工程与食品科学学院,浙江杭州310029

出　　处：《林产化学与工业》2004年第1期95-100,共6页Chemistry and Industry of Forest Products

基　　金：浙江省高校重大科技攻关项目(省长基金9902#);浙江省自然科学基金重点项目(ZA0218)

摘　　要：　综述了竹叶黄酮(EOB-f)与皮肤生理学功效相关的研究进展。最新研究结果表明:1)安全性评价测试显示,EOB-f对皮肤和眼结膜刺激反应均为阴性,无致过敏性;2)化学发光和荧光分析表明,具有可与茶多酚和银杏叶提取物相媲美的抗自由基、抗氧化和抗辐射活性;3)抑菌试验证明,对多种常见感染性细菌有显著的抑制作用;4)抗炎药理试验显示,对巴豆油和二甲苯所致的小鼠耳廓肿胀均具有显著的抑制作用(p<0.01);5)体外细胞培养显示,在0.005%～0.05%浓度内,EOB-f对大鼠皮肤角质形成细胞和成纤维细胞均有显著的增殖作用(p<0.05和p<0.01);对B16黑色素肿瘤细胞的黑色素合成具有显著的抑制作用(p<0.01);能显著降低皮肤角质形成细胞的脂质过氧化产物(MDA)含量,并提高超氧化物歧化酶(SOD)活性(p<0.05)。竹叶黄酮具备了作为一种安全、高效、经济的植物化学素用作抗衰老护肤因子充分和必要的条件,在日用化妆品领域有着广阔的应用前景。The latest researches on the physiological activity of bamboo-leaf-flavonoids(EOB-f) on skin were described as follows:1)Security evaluation tests showed that EOB-f had no off-phenomenon such as pungency and allergy on rat's skin and eye conjunctiva;2)Chemiluminescence and fluorescence analysis showed that EOB-f was of comparative activities with tea polyphenols and the extract of Ginkgo biloba on anti-free radicals,anti-oxidation,and anti-radiation;3)Bacteria inhibition test in vitro approved that EOB-f was capable of inhibiting various strains of infectious bacteria;4)Anti-inflammation tests revealed that EOB-f has obvious inhibiting effect on mice′s auricle swell caused both by croton oil and dimethylbenzene;5)Cell incubation tests in vitro displayed that,within the concentrations of (0.005%) to 0.05%,EOB-f accelerated significantly the proliferation of two kinds of rat skin cells,kerationcytes and fibroblasts,inhibited the pigment synthesis of B_(16) black pigment cancer cell,and depressed(MDA) content and enhanced SOD activity of rat skin kerationcytes.In conclusion,EOB-f possesses the essential and sufficient qualities applicable as a kind of safe,effective and economic plant components for anti-aging factor to protect skin in cosmetic.

关 键 词：竹叶黄酮 抗衰老护肤因子 安全性评价 抑菌试验 抗炎药理试验 化妆品 

分 类 号：TQ658.2[化学工程—精细化工] TQ652