高效毛细管电泳法测定地塞米松磷酸钠脂质体中地塞米松磷酸钠的含量  

HPCE Determination of Dexamethasone Sodium Phosphate in Liposome

作　　者：郭丹[1] 李国锋[1] 杨西晓[1] 侯连兵[1] 

机构地区：[1]第一军医大学南方医院药学部,广州510515

出　　处：《药物分析杂志》2004年第2期117-119,共3页Chinese Journal of Pharmaceutical Analysis

基　　金：广东省自然科学基金(020056);广东省医学科研基金(A2002371)资助

摘　　要：目的:建立高效毛细管电泳法测定地塞米松磷酸钠脂质体中药物含量。方法:毛细管(60 cm×75μm,有效长度53cm);运行缓冲液100 mmol·L-1硼砂(0.1 mol·L-1盐酸调pH 9.2),高压进样5 s,分离电压20 kV,温度为20℃,头孢拉定为内标,检测波长为240 nm。结果:地塞米松磷酸钠在0.78-50 μg·mL-1浓度范围内线性关系良好(r=0.9998),地塞米松磷酸钠的平均回收率为98.5%。结论:本方法准确、简单、快捷,适用于地塞米松磷酸钠脂质体的质量控制。Objective:To establish an HPCE method for the determination of dexamethasone sodium phosphate in liposome. Methods:HPCE was used. The best condition was achieved with a fused silica capillary tube(60 cm ×75μm,effective length of 53 cm) ,100 mmol·L-1 sodium tetraborate decahydrate buffer(pH 9. 2 adjusted by 0. 1 mol ·L-1 hydrochloric acid) ,at a constant voltage of 20 kV and temperature at 20℃Cefradine was used as internal standard. The UV detection wavelength was 240 nm. Results:The calibration curve was linear in the range of 0. 78 - 50μg·mL-1 for dexamethasone sodium phosphate(r= 0. 9998). The average recovery of dexamethasone sodium phosphate was 98. 5%. Conclusion:This method is simple, quick, accurate and the assay result is suitable for the quality control for dexamethasone sodium phosphate in liposome.

关 键 词：高效毛细管电泳法 地塞米松磷酸钠脂质体 地塞米松磷酸钠 含量测定 卵磷脂 胆固醇 

分 类 号：R927.2[医药卫生—药学] R977.11