咔唑-9-乙基氯甲酸酯柱前衍生氨基酸胶束电动色谱分离  

Micellar Electrokinetic Capillary Chromatographic Separation of Amino Acids Derivatized with 2-(9-Carbazole)-Ethyl Chloroformate as a Pre-column Labeling Reagent

作　　者：张琳[1] 尤进茂[2] 平贵臣[1] 张维冰[1] 阎超[1] 张玉奎[1] 

机构地区：[1]中国科学院大连化学物理研究所,辽宁大连116011 [2]曲阜师范大学化学系,山东曲阜273165

出　　处：《色谱》2004年第2期166-169,共4页Chinese Journal of Chromatography

基　　金：国家"十五"攻关资助项目(色谱仪及专用部件的研制与开发)(G010101).

摘　　要：采用一种新型紫外、荧光衍生试剂咔唑 9 乙基氯甲酸酯对9种氨基酸(丝氨酸、苏氨酸、甘氨酸、谷氨酸、天冬氨酸、缬氨酸、异亮氨酸、亮氨酸、苯丙氨酸)进行柱前衍生,采用胶束电动色谱模式在14min内完成分离。分离条件:以pH9 0的20mmol/L硼酸盐 30mmol/L十二烷基硫酸钠(SDS)溶液(含3%(体积分数)乙腈)为缓冲溶液,柱温25℃,分离电压18kV,紫外检测波长214nm。该方法的线性范围为0 025～0 25mmol/L,检出限为2 15～2 46μmol/L。2-(9-Carbazole)-ethyl chloroformate (CEOC) has been used for the pre-column derivatization of (amino) (acids) in micellar electrokinetic capillary chromatography (MEKC). Nine amino acid standards (Ser, Thr, Gly, Glu, Asp, Val, Ile, Leu, Phe) were labeled with CEOC and separated in a buffer system containing 20 (mmol/L) borate-30 (mmol/L) sodium lauryl sulfate (SDS) and 3% acetonitrile at pH 9.0 with an applied voltage of 18 kV and column temperature of 25 ℃. The derivatives were detected at UV 214 nm. Under optimal conditions, this method offers a baseline separation for nine CEOC-derivatized amino acids within 14 min. The linearity range was from 0.025 to 0.25 (mmol/L) and the detection limits were from 2.15 to 2.46 (μmol/L).

关 键 词：咔唑-9-乙基氯甲酸酯 柱前衍生 氨基酸 胶束电动毛细管色谱 分离 

分 类 号：Q517[生物学—生物化学]