短时吸入安氟醚、异氟醚对大鼠边缘系统c-fos基因表达的影响  

The effect of shortly inhaled isoflurane or enflurane on c-fos gene expression of limbic system in rat

作　　者：卢静[1] 戴体俊[1] 曾因明[1] 张励才[1] 

机构地区：[1]徐州医学院江苏省麻醉学重点实验室,徐州221002

出　　处：《中国药理学通报》2004年第3期259-262,共4页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助课题No 39970 71 5;江苏省自然科学基金资助课题BK 2 0 0 1 1 43

摘　　要：目的　探讨短时吸入安氟醚、异氟醚麻醉对大鼠边缘系统c fos原癌基因表达的影响。方法　 18只SD♂大鼠随机均分为 3组 :对照组、安氟醚组、异氟醚组 ,每组 6只。用c fos免疫组化技术观察短时吸入 2 %安氟醚或 2 %异氟醚对大鼠边缘系统c fos原癌基因的表达的影响。结果　安氟醚、异氟醚两组大鼠的杏仁中介核、杏仁内侧核、杏仁基外侧核、外侧僵核、正中视前核、外侧隔核、终纹床核、隔下丘脑核、下丘脑室周核、视上核等 10个核团Fos免疫样 (fos likeimmunoreactive ,FLI)阳性神经元数目均增加。除安氟醚组杏仁内侧核和视上核增加不明显 (P >0 0 5)外 ,差异均有统计学意义 (P <0 0 1)。结论　边缘系统中杏仁中介核、杏仁基外侧核、外侧僵核、正中视前核、外侧隔核、终纹床核、隔下丘脑核、下丘脑室周核等 8个核团可能参与了安氟醚、异氟醚的麻醉诱导过程 。AIMTo investigate the effect of shor tly inhaled isoflurane or isoflurane on c-fos gene expression of limbic system in rat. METHODS18 SD male rats weighing 200～250 g were randomi zed into three groups, control group, isoflurane group, enflurane group.The anim als in enflurane group or isoflurane group breathed 2% enflurane or 2% isofluran e till righing reflex nearly disappear.The animals in control group underwent th e same experimental steps except inhalation of anesthesia.The effect of shortly inhaled isoflurane or enflurane on c-fos gene in limbic system was observed wit h Fos immunohistochemical staining technique. RESULTNumber of FL I positive neurons of the 10 nucleus was increased significantly in limbic syst em by isoflurane, but enflurane only influenced FLI positive neurons expression of 8 out of the 10 nucleus. CONCLUSIONNuclei with significant ch ange of c-fos expression might be related to isoflurane or enflurane anesthes ia induction.

关 键 词：安氟醚 异氟醚 边缘系统 C-FOS基因 麻醉 机制 

分 类 号：R-332[医药卫生] R332.81