ClC-3反义寡核苷酸对ConA诱导的T淋巴细胞增殖的影响  

The effects of ClC-3 antisense oligonucleotide on ConA-induced Tlymphocytes proliferation

作　　者：钱艳[1] 关永源[2] 王冠蕾[2] 杨晓茹[2] 贺华[2] 丘钦英[2] 

机构地区：[1]中山大学中山医学院分子医学研究中心,广州510080 [2]中山大学中山医学院药理学教研室,广州510080

出　　处：《中国药理学通报》2004年第3期314-317,共4页Chinese Pharmacological Bulletin

基　　金：广东省自然科学基金团队资助课题 ( 2 0 0 1 );中华医学基金会基金资助课题(CMB ;No 0 0 730 );博士点基金资助课题;No 2 0 0 2 0 5580 55

摘　　要：目的　研究ClC 3蛋白在T淋巴细胞增殖过程中的作用。方法　采用脂质体转染技术 ,将寡核苷酸导入细胞 ,免疫印迹 (WesternBlot)方法分析ClC 3蛋白表达 ;[3H] TdR参入法观察ClC 3反义寡核苷酸对细胞增殖的作用。结果　①ClC 3反义寡核苷酸浓度依赖性地抑制ConA诱导的ClC 3蛋白的表达 ;②ClC 3反义寡核苷酸浓度依赖性地抑制ConA诱导的T淋巴细胞增殖。结论　ClCAIMTo study the function of ClC-3 protein on the proliferation of human T lymphocyte. METHODSWith western Blot and [ 3H]-thymidine incorporation, the effects of ClC-3 antisense oligonucleotide on ConA induced human T lymphocyte protein expression and proliferation was measured. RESULTSClC-3 antisense oligonucleotide decreased ConA (10 μg·ml -1 )-induced ClC-3 protein expression and proliferation of T lymphocyte in a dose-dependent manner. CONCLUSIONClC-3 protein takes part in the proliferation of human T lymphocytes.

关 键 词：CLC-3 T淋巴细胞 反义寡核苷酸 增殖 

分 类 号：R329.24[医药卫生—人体解剖和组织胚胎学] R329.25[医药卫生—基础医学]